Pitzler, Lena, Auler, Markus, Probst, Kristina, Frie, Christian, Bergmeier, Vera, Holzer, Tatjana, Belluoccio, Daniele, van den Bergen, Jocelyn, Etich, Julia ORCID: 0000-0003-3238-6692, Ehlen, Harald, Zhou, Zhigang, Bielke, Wolfgang, Poschl, Ernst, Paulsson, Mats and Brachvogel, Bent (2016). miR-126-3p Promotes Matrix-Dependent Perivascular Cell Attachment, Migration and Intercellular Interaction. Stem Cells, 34 (5). S. 1297 - 1310. HOBOKEN: WILEY. ISSN 1549-4918

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Abstract

microRNAs (miRNAs) can regulate the interplay between perivascular cells (PVC) and endothelial cells (EC) during angiogenesis, but the relevant PVC-specific miRNAs are not yet defined. Here, we identified miR-126-3p and miR-146a to be exclusively upregulated in PVC upon interaction with EC, determined their influence on the PVC phenotype and elucidate their molecular mechanisms of action. Specifically the increase of miR-126-3p strongly promoted the motility of PVC on the basement membrane-like composite and stabilized networks of EC. Subsequent miRNA target analysis showed that miR-126-3p inhibits SPRED1 and PLK2 expression, induces ERK1/2 phosphorylation and stimulates TLR3 expression to modulate cell-cell and cell-matrix contacts of PVC. Gain of expression experiments in vivo demonstrated that miR-126-3p stimulates PVC coverage of newly formed vessels and transform immature into mature, less permeable vessels. In conclusion we showed that miR-126-3p regulates matrix-dependent PVC migration and intercellular interaction to modulate vascular integrity.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Pitzler, LenaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Auler, MarkusUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Probst, KristinaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Frie, ChristianUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bergmeier, VeraUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Holzer, TatjanaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Belluoccio, DanieleUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
van den Bergen, JocelynUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Etich, JuliaUNSPECIFIEDorcid.org/0000-0003-3238-6692UNSPECIFIED
Ehlen, HaraldUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Zhou, ZhigangUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bielke, WolfgangUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Poschl, ErnstUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Paulsson, MatsUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Brachvogel, BentUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-278081
DOI: 10.1002/stem.2308
Journal or Publication Title: Stem Cells
Volume: 34
Number: 5
Page Range: S. 1297 - 1310
Date: 2016
Publisher: WILEY
Place of Publication: HOBOKEN
ISSN: 1549-4918
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
STEM-CELLS; DIFFERENTIATION; ANGIOGENESIS; MICRORNA-126; ACTIVATION; RNAMultiple languages
Cell & Tissue Engineering; Biotechnology & Applied Microbiology; Oncology; Cell Biology; HematologyMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/27808

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