Universität zu Köln

Chaves, Gustavo, Derst, Christian, Franzen, Arne ORCID: 0000-0002-8731-674X, Mashimo, Yuta ORCID: 0000-0001-6866-1778, Machida, Ryuichiro and Musset, Boris ORCID: 0000-0001-8242-8817 (2016). Identification of an H(V)1 voltage-gated proton channel in insects. FEBS J., 283 (8). S. 1453 - 1465. HOBOKEN: WILEY. ISSN 1742-4658

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Abstract

The voltage-gated proton channel 1 (H(V)1) is an important component of the cellular proton extrusion machinery and is essential for charge compensation during the respiratory burst of phagocytes. H(V)1 has been identified in a wide range of eukaryotes throughout the animal kingdom, with the exception of insects. Therefore, it has been proposed that insects do not possess an H(V)1 channel. In the present study, we report the existence of an H(V)1-type proton channel in insects. We searched insect transcriptome shotgun assembly (TSA) sequence databases and found putative H(V)1 orthologues in various polyneopteran insects. To confirm that these putative H(V)1 orthologues were functional channels, we studied the H(V)1 channel of Nicoletia phytophila (NpH(V)1), an insect of the Zygentoma order, in more detail. NpH(V)1 comprises 239 amino acids and is 33% identical to the human voltage-gated proton channel 1. Patch clamp measurements in a heterologous expression system showed proton selectivity, as well as pH- and voltage-dependent gating. Interestingly, NpH(V)1 shows slightly enhanced pH-dependent gating compared to the human channel. Mutations in the first transmembrane segment at position 66 (Asp66), the presumed selectivity filter, lead to a loss of proton-selective conduction, confirming the importance of this aspartate residue in voltage-gated proton channels. DatabaseNucleotide sequence data have been deposited in the GenBank database under accession number .

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Chaves, Gustavo UNSPECIFIED UNSPECIFIED UNSPECIFIED Derst, Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED Franzen, Arne UNSPECIFIED orcid.org/0000-0002-8731-674X UNSPECIFIED Mashimo, Yuta UNSPECIFIED orcid.org/0000-0001-6866-1778 UNSPECIFIED Machida, Ryuichiro UNSPECIFIED UNSPECIFIED UNSPECIFIED Musset, Boris UNSPECIFIED orcid.org/0000-0001-8242-8817 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-279710
DOI:	10.1111/febs.13680
Journal or Publication Title:	FEBS J.
Volume:	283
Number:	8
Page Range:	S. 1453 - 1465
Date:	2016
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1742-4658
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language HYDROGEN-ION CURRENTS; ALVEOLAR EPITHELIAL-CELLS; SELECTIVITY FILTER; HUMAN-NEUTROPHILS; HUMAN BASOPHILS; BREAST-CANCER; NADPH OXIDASE; H+ CHANNEL; HV1; PH Multiple languages Biochemistry & Molecular Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/27971