Brych, Annika, Mascarenhas, Judita, Jaeger, Elaine, Charkiewicz, Elzbieta, Pokorny, Richard, Boelker, Michael, Doehlemann, Gunther ORCID: 0000-0002-7353-8456 and Batschauer, Alfred (2016). White collar 1-induced photolyase expression contributes to UV-tolerance of Ustilago maydis. MicrobiologyOpen, 5 (2). S. 224 - 244. HOBOKEN: WILEY. ISSN 2045-8827

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Abstract

Ustilago maydis is a phytopathogenic fungus causing corn smut disease. It also is known for its extreme tolerance to UV- and ionizing radiation. It has not been elucidated whether light-sensing proteins, and in particular photolyases play a role in its UV-tolerance. Based on homology analysis, U. maydis has 10 genes encoding putative light-responsive proteins. Four amongst these belong to the cryptochrome/photolyase family (CPF) and one represents a white collar 1 ortholog (wco1). Deletion mutants in the predicted cyclobutane pyrimidine dimer CPD- and (6-4)-photolyase were impaired in photoreactivation. In line with this, in vitro studies with recombinant CPF proteins demonstrated binding of the catalytic FAD cofactor, its photoreduction to fully reduced FADH(-) and repair activity for cyclobutane pyrimidine dimers (CPDs) or (6-4)-photoproducts, respectively. We also investigated the role of Wco1. Strikingly, transcriptional profiling showed 61 genes differentially expressed upon blue light exposure of wild-type, but only eight genes in the wco1 mutant. These results demonstrate that Wco1 is a functional blue light photoreceptor in U. maydis regulating expression of several genes including both photolyases. Finally, we show that the wco1 mutant is less tolerant against UV-B due to its incapability to induce photolyase expression.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Brych, AnnikaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Mascarenhas, JuditaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Jaeger, ElaineUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Charkiewicz, ElzbietaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Pokorny, RichardUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Boelker, MichaelUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Doehlemann, GuntherUNSPECIFIEDorcid.org/0000-0002-7353-8456UNSPECIFIED
Batschauer, AlfredUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-279809
DOI: 10.1002/mbo3.322
Journal or Publication Title: MicrobiologyOpen
Volume: 5
Number: 2
Page Range: S. 224 - 244
Date: 2016
Publisher: WILEY
Place of Publication: HOBOKEN
ISSN: 2045-8827
Language: English
Faculty: Faculty of Mathematics and Natural Sciences
Divisions: Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
BLUE-LIGHT PHOTORECEPTORS; GENE REPLACEMENT MUTANTS; NEUROSPORA-CRASSA; ASPERGILLUS-NIDULANS; ARABIDOPSIS-THALIANA; SEXUAL DEVELOPMENT; DNA PHOTOLYASE; PATHOGENIC DEVELOPMENT; ESCHERICHIA-COLI; PHYTOCHROME FPHAMultiple languages
MicrobiologyMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/27980

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