Universität zu Köln

Prantl, Lukas, Schreml, Julia, Gehmert, Sebastian ORCID: 0000-0002-4794-0660, Klein, Silvan, Bai, Xiaowen, Zeitler, Katharina, Schreml, Stephan ORCID: 0000-0002-2820-1942, Alt, Eckhard, Gehmert, Sanga and Felthaus, Oliver (2016). Transcription Profile in Sporadic Multiple Symmetric Lipomatosis Reveals Differential Expression at the Level of Adipose Tissue-Derived Stem Cells. Plast. Reconstr. Surg., 137 (4). S. 1181 - 1191. PHILADELPHIA: LIPPINCOTT WILLIAMS & WILKINS. ISSN 1529-4242

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Abstract

Background: The cause of the rare fat distribution disorder multiple symmetric lipomatosis is unknown. Independent reports suggest a higher proliferative activity, hormone resistance, and involvement of mitochondrial function in the disease. Methods: The authors performed morphologic comparison of affected and unaffected tissues in five unrelated patients and generated adipose-derived stem cell cultures from the tissue samples and characterized them as a possible cellular model of multiple symmetric lipomatosis evolution. The authors investigated proliferative activity and the expression of genes relevant to disease processes. Results: There was no difference in the morphologic appearance and the surface marker profile. Stem cells from lipomatous tissue showed significantly higher proliferative activity. Polymerase chain reaction arrays showed marked changes in genes associated with proliferation, hormonal regulation, and mitochondria. The authors show that multiple symmetric lipomatosis tissue is morphologically and histologically different from regular subcutaneous fat. Conclusions: This study indicates an involvement of mesenchymal stem cells in the pathogenesis of multiple symmetric lipomatosis and that the evolution of multiple symmetric lipomatosis tissue is a process driven by an inherent defect of the respective cell clone(s). Further molecular genetics and functional analysis will be required to unravel the pathogenetic mechanism underlying the derailment in fat cell metabolism and proliferation. Here, the authors show for the first time that adipose-derived stem cells exhibit many characteristics previously described for native multiple symmetric lipomatosis fat tissue and propose that they are therefore an excellent tool for further functional investigations in multiple symmetric lipomatosis and other disorders of the fat tissue. CLINICAL QUESTION/LEVEL OF EVIDENCE: Risk, V.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Prantl, Lukas UNSPECIFIED UNSPECIFIED UNSPECIFIED Schreml, Julia UNSPECIFIED UNSPECIFIED UNSPECIFIED Gehmert, Sebastian UNSPECIFIED orcid.org/0000-0002-4794-0660 UNSPECIFIED Klein, Silvan UNSPECIFIED UNSPECIFIED UNSPECIFIED Bai, Xiaowen UNSPECIFIED UNSPECIFIED UNSPECIFIED Zeitler, Katharina UNSPECIFIED UNSPECIFIED UNSPECIFIED Schreml, Stephan UNSPECIFIED orcid.org/0000-0002-2820-1942 UNSPECIFIED Alt, Eckhard UNSPECIFIED UNSPECIFIED UNSPECIFIED Gehmert, Sanga UNSPECIFIED UNSPECIFIED UNSPECIFIED Felthaus, Oliver UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-279847
DOI:	10.1097/PRS.0000000000002013
Journal or Publication Title:	Plast. Reconstr. Surg.
Volume:	137
Number:	4
Page Range:	S. 1181 - 1191
Date:	2016
Publisher:	LIPPINCOTT WILLIAMS & WILKINS
Place of Publication:	PHILADELPHIA
ISSN:	1529-4242
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ADRENERGIC-STIMULATED LIPOLYSIS; MESSENGER-RNA EXPRESSION; UNCOUPLING PROTEIN; BROWN FAT; METABOLISM; DISEASE; OBESE; INTERLEUKIN-6; ACCUMULATION; LIPOSUCTION Multiple languages Surgery Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/27984