Michalk, Maike, Meinrath, Jeannine, Kuenstlinger, Helen, Koitzsch, Ulrike, Drebber, Uta, Merkelbach-Bruse, Sabine, Bollschweiler, Elfriede, Kloth, Michael, Hartmann, Wolfgang ORCID: 0000-0002-7609-5021, Hoelscher, Arnulf, Quaas, Alexander, Grimminger, Peter P. and Odenthal, Margarete (2016). MDM2 gene amplification in esophageal carcinoma. Oncol. Rep., 35 (4). S. 2223 - 2228. ATHENS: SPANDIDOS PUBL LTD. ISSN 1791-2431

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Abstract

Esophageal cancer (EC) is one of the most common malignancies diagnosed in the Western world with an increasing incidence noted for esophageal adenocarcinoma (EAC). Despite improvements in staging, surgical procedures and postoperative treatments, the overall survival of patients with EC remains low. Murine double minute-2 (MDM2) acts as an oncogene by inducing the degradation of the tumor-suppressor protein TP53. In order to evaluate the MDM2 gene amplification status in EAC and squamous cell carcinoma (SCC), we established a quantitative PCR (qPCR) assay, screening a total of 127 esophageal carcinoma cases for MDM2 amplification. Esophageal carcinoma cases with enhanced MDM2 gene copy numbers were further analyzed by fluorescence in situ hybridisation (FISH) and MDM2 immunostaining. Among a total of 23 specimens (18%), identified by qPCR to possess elevated MDM2 gene copy numbers, one third (6.3%) showed a cluster-like fluorescence pattern by FISH analyses and marked MDM2 protein immunostaining. MDM2 gene amplifications did not correlate with the occurrence of TP53 mutations. Due to the high therapeutic relevance of MDM2 overexpression, but the high cost of FISH, we suggest a primary screening of MDM2 copy number variations by qPCR, followed by detailed FISH analysis of the identified ECs.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Michalk, MaikeUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Meinrath, JeannineUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kuenstlinger, HelenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Koitzsch, UlrikeUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Drebber, UtaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Merkelbach-Bruse, SabineUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bollschweiler, ElfriedeUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kloth, MichaelUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hartmann, WolfgangUNSPECIFIEDorcid.org/0000-0002-7609-5021UNSPECIFIED
Hoelscher, ArnulfUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Quaas, AlexanderUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Grimminger, Peter P.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Odenthal, MargareteUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-280671
DOI: 10.3892/or.2016.4578
Journal or Publication Title: Oncol. Rep.
Volume: 35
Number: 4
Page Range: S. 2223 - 2228
Date: 2016
Publisher: SPANDIDOS PUBL LTD
Place of Publication: ATHENS
ISSN: 1791-2431
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
SQUAMOUS-CELL CARCINOMA; P53 MUTATION; CANCER; PROTEIN; ADENOCARCINOMA; EXPRESSION; SURVIVAL; TP53; POLYMORPHISMS; ACCUMULATIONMultiple languages
OncologyMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/28067

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