Universität zu Köln

Schneider, Anja, Steinberger, Iris, Herdean, Andrei ORCID: 0000-0003-2143-0213, Gandini, Chiara ORCID: 0000-0001-5536-3608, Eisenhut, Marion, Kurz, Samantha, Morper, Anna, Hoecker, Natalie, Ruehle, Thilo, Labs, Mathias ORCID: 0000-0003-2104-2763, Fluegge, Ulf-Ingo, Geimer, Stefan, Schmidt, Sidsel Birkelund ORCID: 0000-0002-4193-4454, Husted, Soren ORCID: 0000-0003-2020-1902, Weber, Andreas P. M., Spetea, Cornelia and Leister, Dario ORCID: 0000-0003-1897-8421 (2016). The Evolutionarily Conserved Protein PHOTOSYNTHESIS AFFECTED MUTANT71 Is Required for Efficient Manganese Uptake at the Thylakoid Membrane in Arabidopsis. Plant Cell, 28 (4). S. 892 - 911. ROCKVILLE: AMER SOC PLANT BIOLOGISTS. ISSN 1531-298X

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Abstract

In plants, algae, and cyanobacteria, photosystem II (PSII) catalyzes the light-driven oxidation of water. The oxygen-evolving complex of PSII is a Mn4CaO5 cluster embedded in a well-defined protein environment in the thylakoid membrane. However, transport of manganese and calcium into the thylakoid lumen remains poorly understood. Here, we show that Arabidopsis thaliana PHOTOSYNTHESIS AFFECTED MUTANT71 (PAM71) is an integral thylakoid membrane protein involved in Mn2+ and Ca2+ homeostasis in chloroplasts. This protein is required for normal operation of the oxygen-evolving complex (as evidenced by oxygen evolution rates) and for manganese incorporation. Manganese binding to PSII was severely reduced in pam71 thylakoids, particularly in PSII supercomplexes. In cation partitioning assays with intact chloroplasts, Mn2+ and Ca2+ ions were differently sequestered in pam71, with Ca2+ enriched in pam71 thylakoids relative to the wild type. The changes in Ca2+ homeostasis were accompanied by an increased contribution of the transmembrane electrical potential to the proton motive force across the thylakoid membrane. PSII activity in pam71 plants and the corresponding Chlamydomonas reinhardtii mutant cgld1 was restored by supplementation with Mn2+, but not Ca2+. Furthermore, PAM71 suppressed the Mn2+-sensitive phenotype of the yeast mutant Delta pmr1. Therefore, PAM71 presumably functions in Mn2+ uptake into thylakoids to ensure optimal PSII performance.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Schneider, Anja UNSPECIFIED UNSPECIFIED UNSPECIFIED Steinberger, Iris UNSPECIFIED UNSPECIFIED UNSPECIFIED Herdean, Andrei UNSPECIFIED orcid.org/0000-0003-2143-0213 UNSPECIFIED Gandini, Chiara UNSPECIFIED orcid.org/0000-0001-5536-3608 UNSPECIFIED Eisenhut, Marion UNSPECIFIED UNSPECIFIED UNSPECIFIED Kurz, Samantha UNSPECIFIED UNSPECIFIED UNSPECIFIED Morper, Anna UNSPECIFIED UNSPECIFIED UNSPECIFIED Hoecker, Natalie UNSPECIFIED UNSPECIFIED UNSPECIFIED Ruehle, Thilo UNSPECIFIED UNSPECIFIED UNSPECIFIED Labs, Mathias UNSPECIFIED orcid.org/0000-0003-2104-2763 UNSPECIFIED Fluegge, Ulf-Ingo UNSPECIFIED UNSPECIFIED UNSPECIFIED Geimer, Stefan UNSPECIFIED UNSPECIFIED UNSPECIFIED Schmidt, Sidsel Birkelund UNSPECIFIED orcid.org/0000-0002-4193-4454 UNSPECIFIED Husted, Soren UNSPECIFIED orcid.org/0000-0003-2020-1902 UNSPECIFIED Weber, Andreas P. M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Spetea, Cornelia UNSPECIFIED UNSPECIFIED UNSPECIFIED Leister, Dario UNSPECIFIED orcid.org/0000-0003-1897-8421 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-280697
DOI:	10.1105/tpc.15.00812
Journal or Publication Title:	Plant Cell
Volume:	28
Number:	4
Page Range:	S. 892 - 911
Date:	2016
Publisher:	AMER SOC PLANT BIOLOGISTS
Place of Publication:	ROCKVILLE
ISSN:	1531-298X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language EVOLVING PHOTOSYSTEM-II; KDA EXTRINSIC PROTEIN; CHLOROPHYLL-FLUORESCENCE; SECRETORY PATHWAY; OXYGEN EVOLUTION; REDOX REGULATION; CALCIUM-BINDING; GAMMA-SUBUNIT; SHORT-TERM; THALIANA Multiple languages Biochemistry & Molecular Biology; Plant Sciences; Cell Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/28069