Universität zu Köln

Gromadzka, Agnieszka M., Steckelberg, Anna-Lena ORCID: 0000-0001-7988-9946, Singh, Kusum K., Hofmann, Kay and Gehring, Niels H. ORCID: 0000-0001-7792-1164 (2016). A short conserved motif in ALYREF directs cap- and EJC-dependent assembly of export complexes on spliced mRNAs. Nucleic Acids Res., 44 (5). S. 2348 - 2362. OXFORD: OXFORD UNIV PRESS. ISSN 1362-4962

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Abstract

The export of messenger RNAs (mRNAs) is the final of several nuclear posttranscriptional steps of gene expression. The formation of export-competent mRNPs involves the recruitment of export factors that are assumed to facilitate transport of the mature mRNAs. Using in vitro splicing assays, we show that a core set of export factors, including ALYREF, UAP56 and DDX39, readily associate with the spliced RNAs in an EJC (exon junction complex)- and cap-dependent manner. In order to elucidate how ALYREF and other export adaptors mediate mRNA export, we conducted a computational analysis and discovered four short, conserved, linear motifs present in RNA-binding proteins. We show that mutation in one of the new motifs (WxHD) in an unstructured region of ALYREF reduced RNA binding and abolished the interaction with eIF4A3 and CBP80. Additionally, the mutation impaired proper localization to nuclear speckles and export of a spliced reporter mRNA. Our results reveal important details of the orchestrated recruitment of export factors during the formation of export competent mRNPs.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Gromadzka, Agnieszka M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Steckelberg, Anna-Lena UNSPECIFIED orcid.org/0000-0001-7988-9946 UNSPECIFIED Singh, Kusum K. UNSPECIFIED UNSPECIFIED UNSPECIFIED Hofmann, Kay UNSPECIFIED UNSPECIFIED UNSPECIFIED Gehring, Niels H. UNSPECIFIED orcid.org/0000-0001-7792-1164 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-281172
DOI:	10.1093/nar/gkw009
Journal or Publication Title:	Nucleic Acids Res.
Volume:	44
Number:	5
Page Range:	S. 2348 - 2362
Date:	2016
Publisher:	OXFORD UNIV PRESS
Place of Publication:	OXFORD
ISSN:	1362-4962
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Institute for Genetics
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language EXON JUNCTION COMPLEX; NUCLEAR EXPORT; BINDING PROTEINS; MAMMALIAN-CELLS; LINEAR MOTIFS; TREX COMPLEX; IN-VITRO; UAP56; RECRUITMENT; TRANSCRIPTION Multiple languages Biochemistry & Molecular Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/28117