Al-Emran, Hassan M., Hahn, Andreas ORCID: 0000-0002-5157-8369, Baum, Jana, Espinoza, Ligia Maria Cruz, Deerin, Jessica, Im, Justin, Ibrango, Samuel, Kabore, Leon Parfait, von Kalckreuth, Vera, Konings, Frank, Marks, Florian ORCID: 0000-0002-6043-7170, Sampo, Emmanuel, Panzner, Ursula, Park, Se Eun, Pak, Gi Deok, Schuett-Gerowitt, Heidi, Vinnemeier, Christof David, Warren, Michelle and Soura, Abdramane Bassiahi (2016). Diagnosing Salmonella enterica Serovar Typhi Infections by Polymerase Chain Reaction Using EDTA Blood Samples of Febrile Patients From Burkina Faso. Clin. Infect. Dis., 62. S. S37 - 5. CARY: OXFORD UNIV PRESS INC. ISSN 1537-6591

Full text not available from this repository.

Abstract

Methods.aEuro integral From April 2012 to September 2013, typhoid fever surveillance was conducted in Polesgo and Nioko, 2 dry slum areas in Ouagadougou, Burkina Faso. Blood culture was performed for febrile patients using an automated blood culture system. Additional blood was collected in EDTA tubes from those patients and preserved at -80A degrees C. DNA was extracted from EDTA blood and PCR was performed to identify presence of S. Typhi. Randomly selected PCR products were further sequenced to identify S. Typhi-specific amplicons. Results.aEuro integral Of 1674 patients, S. Typhi was isolated from 18 (1.1%) individuals by blood culture. EDTA blood was collected from 1578 patients, of which 298 EDTA samples were tested by PCR. Salmonella Typhi-specific DNA was identified in 44 (14.8%) samples. The sensitivity of S. Typhi-specific PCR from EDTA blood was 89% (74%-100%) among the blood culture-positive cases. Sixteen S. Typhi-positive PCR products were sequenced, and 13 retrieved the sequence of a S. Typhi-specific amplicon. Conclusions.aEuro integral These findings suggest that blood culture-based diagnoses of S. Typhi underestimate the burden of typhoid fever in Burkina Faso. PCR could be considered as an alternative method for the identification and diagnosis of S. Typhi in blood samples.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Al-Emran, Hassan M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hahn, AndreasUNSPECIFIEDorcid.org/0000-0002-5157-8369UNSPECIFIED
Baum, JanaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Espinoza, Ligia Maria CruzUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Deerin, JessicaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Im, JustinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ibrango, SamuelUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kabore, Leon ParfaitUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
von Kalckreuth, VeraUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Konings, FrankUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Marks, FlorianUNSPECIFIEDorcid.org/0000-0002-6043-7170UNSPECIFIED
Sampo, EmmanuelUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Panzner, UrsulaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Park, Se EunUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Pak, Gi DeokUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schuett-Gerowitt, HeidiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Vinnemeier, Christof DavidUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Warren, MichelleUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Soura, Abdramane BassiahiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-281311
DOI: 10.1093/cid/civ770
Journal or Publication Title: Clin. Infect. Dis.
Volume: 62
Page Range: S. S37 - 5
Date: 2016
Publisher: OXFORD UNIV PRESS INC
Place of Publication: CARY
ISSN: 1537-6591
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
BONE-MARROW; CULTURE; FEVER; PCR; BACTERIA; CHILDRENMultiple languages
Immunology; Infectious Diseases; MicrobiologyMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/28131

Downloads

Downloads per month over past year

Altmetric

Export

Actions (login required)

View Item View Item