Universität zu Köln

Burnouf, Sylvie, Groenke, Sebastian, Augustin, Hrvoje, Dols, Jacqueline, Gorsky, Marianna Karina, Werner, Jennifer, Kerr, Fiona ORCID: 0000-0003-3097-1951, Alic, Nazif ORCID: 0000-0003-0356-6600, Martinez, Pedro and Partridge, Linda ORCID: 0000-0001-9615-0094 (2016). Deletion of endogenous Tau proteins is not detrimental in Drosophila. Sci Rep, 6. LONDON: NATURE PUBLISHING GROUP. ISSN 2045-2322

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Abstract

Human Tau (hTau) is a highly soluble and natively unfolded protein that binds to microtubules within neurons. Its dysfunction and aggregation into insoluble paired helical filaments is involved in the pathogenesis of Alzheimer's disease (AD), constituting, together with accumulated beta-amyloid (A beta) peptides, a hallmark of the disease. Deciphering both the loss-of-function and toxic gain-of-function of hTau proteins is crucial to further understand the mechanisms leading to neurodegeneration in AD. As the fruit fly Drosophila melanogaster expresses Tau proteins (dTau) that are homologous to hTau, we aimed to better comprehend dTau functions by generating a specific tau knock-out (KO) fly line using homologous recombination. We observed that the specific removal of endogenous dTau proteins did not lead to overt, macroscopic phenotypes in flies. Indeed, survival, climbing ability and neuronal function were unchanged in tau KO flies. In addition, we did not find any overt positive or negative effect of dTau removal on human A beta-induced toxicity. Altogether, our results indicate that the absence of dTau proteins has no major functional impact on flies, and suggests that our tau KO strain is a relevant model to further investigate the role of dTau proteins in vivo, thereby giving additional insights into hTau functions.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Burnouf, Sylvie UNSPECIFIED UNSPECIFIED UNSPECIFIED Groenke, Sebastian UNSPECIFIED UNSPECIFIED UNSPECIFIED Augustin, Hrvoje UNSPECIFIED UNSPECIFIED UNSPECIFIED Dols, Jacqueline UNSPECIFIED UNSPECIFIED UNSPECIFIED Gorsky, Marianna Karina UNSPECIFIED UNSPECIFIED UNSPECIFIED Werner, Jennifer UNSPECIFIED UNSPECIFIED UNSPECIFIED Kerr, Fiona UNSPECIFIED orcid.org/0000-0003-3097-1951 UNSPECIFIED Alic, Nazif UNSPECIFIED orcid.org/0000-0003-0356-6600 UNSPECIFIED Martinez, Pedro UNSPECIFIED UNSPECIFIED UNSPECIFIED Partridge, Linda UNSPECIFIED orcid.org/0000-0001-9615-0094 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-281449
DOI:	10.1038/srep23102
Journal or Publication Title:	Sci Rep
Volume:	6
Date:	2016
Publisher:	NATURE PUBLISHING GROUP
Place of Publication:	LONDON
ISSN:	2045-2322
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language BETA TOXICITY; PHOSPHORYLATION; MODELS; PAR-1; DEGENERATION; EXPRESSION; DEFICITS; FIBER; APP Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/28144