Universität zu Köln

Maass, Tobias, Bayley, Christopher P., Morgelin, Matthias, Lettmann, Sandra, Bonaldo, Paolo, Paulsson, Mats, Baldock, Clair ORCID: 0000-0003-3497-1959 and Wagener, Raimund (2016). Heterogeneity of Collagen VI Microfibrils STRUCTURAL ANALYSIS OF NON-COLLAGENOUS REGIONS. J. Biol. Chem., 291 (10). S. 5247 - 5259. BETHESDA: AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC. ISSN 1083-351X

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Abstract

Collagen VI, a collagen with uncharacteristically large N- and C-terminal non-collagenous regions, forms a distinct microfibrillar network in most connective tissues. It was long considered to consist of three genetically distinct chains (1, 2, and 3). Intracellularly, heterotrimeric molecules associate to form dimers and tetramers, which are then secreted and assembled to microfibrils. The identification of three novel long collagen VI chains, 4, 5, and 6, led to the question if and how these may substitute for the long 3 chain in collagen VI assembly. Here, we studied structural features of the novel long chains and analyzed the assembly of these into tetramers and microfibrils. N- and C-terminal globular regions of collagen VI were recombinantly expressed and studied by small angle x-ray scattering (SAXS). Ab initio models of the N-terminal globular regions of the 4, 5, and 6 chains showed a C-shaped structure similar to that found for the 3 chain. Single particle EM nanostructure of the N-terminal globular region of the 4 chain confirmed the C-shaped structure revealed by SAXS. Immuno-EM of collagen VI extracted from tissue revealed that like the 3 chain the novel long chains assemble to homotetramers that are incorporated into mixed microfibrils. Moreover, SAXS models of the C-terminal globular regions of the 1, 2, 4, and 6 chains were generated. Interestingly, the 1, 2, and 4 C-terminal globular regions dimerize. These self-interactions may play a role in tetramer formation.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Maass, Tobias UNSPECIFIED UNSPECIFIED UNSPECIFIED Bayley, Christopher P. UNSPECIFIED UNSPECIFIED UNSPECIFIED Morgelin, Matthias UNSPECIFIED UNSPECIFIED UNSPECIFIED Lettmann, Sandra UNSPECIFIED UNSPECIFIED UNSPECIFIED Bonaldo, Paolo UNSPECIFIED UNSPECIFIED UNSPECIFIED Paulsson, Mats UNSPECIFIED UNSPECIFIED UNSPECIFIED Baldock, Clair UNSPECIFIED orcid.org/0000-0003-3497-1959 UNSPECIFIED Wagener, Raimund UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-281682
DOI:	10.1074/jbc.M115.705160
Journal or Publication Title:	J. Biol. Chem.
Volume:	291
Number:	10
Page Range:	S. 5247 - 5259
Date:	2016
Publisher:	AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Place of Publication:	BETHESDA
ISSN:	1083-351X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SMALL-ANGLE SCATTERING; BIOLOGICAL MACROMOLECULES; BETHLEM MYOPATHY; ALPHA-3 CHAIN; DOMAIN; RESOLUTION; PROTEOGLYCAN; DEFICIENCY; MUTATIONS; SECRETION Multiple languages Biochemistry & Molecular Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/28168