Schulte, Kathrin, Pawlowski, Nikolaus, Faelber, Katja, Froehlich, Chris, Howard, Jonathan ORCID: 0000-0003-2756-5143 and Daumke, Oliver (2016). The immunity-related GTPase Irga6 dimerizes in a parallel head-to-head fashion. BMC Biol., 14. LONDON: BMC. ISSN 1741-7007
Full text not available from this repository.Abstract
Background: The immunity-related GTPases (IRGs) constitute a powerful cell-autonomous resistance system against several intracellular pathogens. Irga6 is a dynamin-like protein that oligomerizes at the parasitophorous vacuolar membrane (PVM) of Toxoplasma gondii leading to its vesiculation. Based on a previous biochemical analysis, it has been proposed that the GTPase domains of Irga6 dimerize in an antiparallel fashion during oligomerization. Results: We determined the crystal structure of an oligomerization-impaired Irga6 mutant bound to a non-hydrolyzable GTP analog. Contrary to the previous model, the structure shows that the GTPase domains dimerize in a parallel fashion. The nucleotides in the center of the interface participate in dimerization by forming symmetric contacts with each other and with the switch I region of the opposing Irga6 molecule. The latter contact appears to activate GTP hydrolysis by stabilizing the position of the catalytic glutamate 106 in switch I close to the active site. Further dimerization contacts involve switch II, the G4 helix and the trans stabilizing loop. Conclusions: The Irga6 structure features a parallel GTPase domain dimer, which appears to be a unifying feature of all dynamin and septin superfamily members. This study contributes important insights into the assembly and catalytic mechanisms of IRG proteins as prerequisite to understand their anti-microbial action.
Item Type: | Journal Article | ||||||||||||||||||||||||||||
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URN: | urn:nbn:de:hbz:38-281786 | ||||||||||||||||||||||||||||
DOI: | 10.1186/s12915-016-0236-7 | ||||||||||||||||||||||||||||
Journal or Publication Title: | BMC Biol. | ||||||||||||||||||||||||||||
Volume: | 14 | ||||||||||||||||||||||||||||
Date: | 2016 | ||||||||||||||||||||||||||||
Publisher: | BMC | ||||||||||||||||||||||||||||
Place of Publication: | LONDON | ||||||||||||||||||||||||||||
ISSN: | 1741-7007 | ||||||||||||||||||||||||||||
Language: | English | ||||||||||||||||||||||||||||
Faculty: | Unspecified | ||||||||||||||||||||||||||||
Divisions: | Unspecified | ||||||||||||||||||||||||||||
Subjects: | no entry | ||||||||||||||||||||||||||||
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Refereed: | Yes | ||||||||||||||||||||||||||||
URI: | http://kups.ub.uni-koeln.de/id/eprint/28178 |
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