Universität zu Köln

Breloy, Isabelle, Schwientek, Tilo, Althoff, Deborah, Holz, Marvin, Koppen, Tim, Krupa, Angelika and Hanisch, Franz-Georg (2016). Functional Analysis of the Glucuronyltransferases GlcAT-P and GlcAT-S of Drosophila melanogaster: Distinct Activities towards the O-linked T-antigen. Biomolecules, 6 (1). BASEL: MDPI. ISSN 2218-273X

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Abstract

The Drosophila melanogaster glucuronyltransferases dGlcAT-S and dGlcAT-P were reported to be expressed ubiquitously and results of in vitro activity assays indicate a functional redundancy. We analyzed both transferases in vivo and in vitro and could show significant differences in their activity towards N-and O-glycoproteins in vivo. While GlcAT-P is able to use N-linked N-acetyllactosamine chains and the O-linked T-antigen as a substrate to form non-sulfated HNK1- (GlcA beta 1-3Gal beta 1-4GlcNAc beta 1-) and glucuronyl-T-antigens in vivo, GlcAT-S adds glucuronic acid only to N-linked chains, thereby synthesizing only the non-sulfated HNK1-antigen.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Breloy, Isabelle UNSPECIFIED UNSPECIFIED UNSPECIFIED Schwientek, Tilo UNSPECIFIED UNSPECIFIED UNSPECIFIED Althoff, Deborah UNSPECIFIED UNSPECIFIED UNSPECIFIED Holz, Marvin UNSPECIFIED UNSPECIFIED UNSPECIFIED Koppen, Tim UNSPECIFIED UNSPECIFIED UNSPECIFIED Krupa, Angelika UNSPECIFIED UNSPECIFIED UNSPECIFIED Hanisch, Franz-Georg UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-282376
DOI:	10.3390/biom6010008
Journal or Publication Title:	Biomolecules
Volume:	6
Number:	1
Date:	2016
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	2218-273X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language HNK-1 CARBOHYDRATE; MOUSE-BRAIN; EXPRESSION; GLYCANS; CELLS; DIFFERENTIATION; GLYCOPROTEINS; GLYCOMICS; DOMAINS; EPITOPE Multiple languages Biochemistry & Molecular Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/28237