Universität zu Köln

Weseslindtner, Lukas ORCID: 0000-0001-8950-937X, Macheleidt, Iris, Eischeid, Hannah, Strassl, Robert, Hofer, Harald, Popow-Kraupp, Theresia, Dienes, Hans-Peter, Holzmann, Heidemarie and Odenthal, Margarete (2016). Micro RNAs mir-106a, mir-122 and mir-197 are increased in severe acute viral hepatitis with coagulopathy. Liver Int., 36 (3). S. 353 - 361. HOBOKEN: WILEY. ISSN 1478-3231

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Abstract

Background & AimsThe severity of acute viral hepatitis, which may be caused by several distinct viruses, varies among individual patients. In rare cases, severe hepatic injury with sudden loss of liver function may occur, which is clinically indicated by the occurrence of coagulopathy or encephalopathy. As the molecular mechanisms of this liver injury are largely unknown, we investigated extracellular micro RNA (miRNA) profiles in 54 patients acutely infected with one of four different hepatotropic viruses, in order to identify those miRNAs which indicate severe viral hepatitis associated with coagulopathy. MethodsFirst, the profile of miRNAs was extensively analysed using a microarray-based approach in highly characterized 24 patients, matched in terms of sex, age and level of liver enzymes, as well as in three healthy controls. The cohort included samples from 18 patients with moderate and six individuals with severe hepatitis, indicated by abnormal prothrombin time and higher alanine aminotransferase and bilirubin levels. miRNAs found to be upregulated in severe hepatitis were then quantified by real-time PCR in the expanded cohort of 54 patients. ResultsComprehensive microarray-based miRNA profiling identified upregulation of mir-106a, mir-122 and mir-197 in patients with severe acute viral hepatitis with coagulopathy, as compared to patients who did not develop coagulopathy. mir-106a, mir-122 and mir-197 were then proven to be significantly upregulated in patients with severe acute viral hepatitis by quantitative real-time PCR (P < 0.01, Mann-Whitney U-test). Conclusionsmir-106a, mir-122 and mir-197 could be potential markers for severe acute viral hepatitis associated with coagulopathy.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Weseslindtner, Lukas UNSPECIFIED orcid.org/0000-0001-8950-937X UNSPECIFIED Macheleidt, Iris UNSPECIFIED UNSPECIFIED UNSPECIFIED Eischeid, Hannah UNSPECIFIED UNSPECIFIED UNSPECIFIED Strassl, Robert UNSPECIFIED UNSPECIFIED UNSPECIFIED Hofer, Harald UNSPECIFIED UNSPECIFIED UNSPECIFIED Popow-Kraupp, Theresia UNSPECIFIED UNSPECIFIED UNSPECIFIED Dienes, Hans-Peter UNSPECIFIED UNSPECIFIED UNSPECIFIED Holzmann, Heidemarie UNSPECIFIED UNSPECIFIED UNSPECIFIED Odenthal, Margarete UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-283394
DOI:	10.1111/liv.12961
Journal or Publication Title:	Liver Int.
Volume:	36
Number:	3
Page Range:	S. 353 - 361
Date:	2016
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1478-3231
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language B-VIRUS; CIRCULATING MICRORNAS; FULMINANT-HEPATITIS; EXPRESSION PROFILE; SERUM; PLASMA; CANCER; BIOMARKERS; INFECTION; DIAGNOSE Multiple languages Gastroenterology & Hepatology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/28339