Beil-Wagner, Jane ORCID: 0000-0003-1853-8303, Doessinger, Georg, Schober, Kilian ORCID: 0000-0001-9323-9472, vom Berg, Johannes, Tresch, Achim, Grandl, Martina, Palle, Pushpalatha, Mair, Florian ORCID: 0000-0001-6732-5449, Gerhard, Markus ORCID: 0000-0001-9110-3950, Becher, Burkhard ORCID: 0000-0002-1541-7867, Busch, Dirk H. and Buch, Thorsten ORCID: 0000-0002-2236-9074 (2016). T cell-specific inactivation of mouse CD2 by CRISPR/Cas9. Sci Rep, 6. LONDON: NATURE PUBLISHING GROUP. ISSN 2045-2322

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Abstract

The CRISPR/Cas9 system can be used to mutate target sequences by introduction of double-strand breaks followed by imprecise repair. To test its use for conditional gene editing we generated mice transgenic for CD4 promoter-driven Cas9 combined with guide RNA targeting CD2. We found that within CD4(+) and CD8(+) lymphocytes from lymph nodes and spleen 1% and 0.6% were not expressing CD2, respectively. T cells lacking CD2 carryied mutations, which confirmed that Cas9 driven by cell-type specific promoters can edit genes in the mouse and may thus allow targeted studies of gene function in vivo.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Beil-Wagner, JaneUNSPECIFIEDorcid.org/0000-0003-1853-8303UNSPECIFIED
Doessinger, GeorgUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schober, KilianUNSPECIFIEDorcid.org/0000-0001-9323-9472UNSPECIFIED
vom Berg, JohannesUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Tresch, AchimUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Grandl, MartinaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Palle, PushpalathaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Mair, FlorianUNSPECIFIEDorcid.org/0000-0001-6732-5449UNSPECIFIED
Gerhard, MarkusUNSPECIFIEDorcid.org/0000-0001-9110-3950UNSPECIFIED
Becher, BurkhardUNSPECIFIEDorcid.org/0000-0002-1541-7867UNSPECIFIED
Busch, Dirk H.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Buch, ThorstenUNSPECIFIEDorcid.org/0000-0002-2236-9074UNSPECIFIED
URN: urn:nbn:de:hbz:38-284316
DOI: 10.1038/srep21377
Journal or Publication Title: Sci Rep
Volume: 6
Date: 2016
Publisher: NATURE PUBLISHING GROUP
Place of Publication: LONDON
ISSN: 2045-2322
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
ZINC-FINGER NUCLEASES; GENE; RNA; MICE; CAS9; DNA; CRISPR-CAS9; ENDONUCLEASE; SYSTEMS; RECOMBINATIONMultiple languages
Multidisciplinary SciencesMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/28431

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