Boddey, Justin A., O'Neill, Matthew T., Lopaticki, Sash, Carvalho, Teresa G., Hodder, Anthony N., Nebl, Thomas ORCID: 0000-0003-4003-9340, Wawra, Stephan ORCID: 0000-0001-8555-1618, van West, Pieter ORCID: 0000-0002-0767-6017, Ebrahimzadeh, Zeinab ORCID: 0000-0003-2972-4515, Richard, Dave, Flemming, Sven ORCID: 0000-0001-6461-5870, Spielmann, Tobias, Przyborski, Jude, Babon, Jeff J. and Cowman, Alan F. (2016). Export of malaria proteins requires co-translational processing of the PEXEL motif independent of phosphatidylinositol-3-phosphate binding. Nat. Commun., 7. LONDON: NATURE PUBLISHING GROUP. ISSN 2041-1723

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Abstract

Plasmodium falciparum exports proteins into erythrocytes using the Plasmodium export element (PEXEL) motif, which is cleaved in the endoplasmic reticulum (ER) by plasmepsin V (PMV). A recent study reported that phosphatidylinositol-3-phosphate (PI(3)P) concentrated in the ER binds to PEXEL motifs and is required for export independent of PMV, and that PEXEL motifs are functionally interchangeable with RxLR motifs of oomycete effectors. Here we show that the PEXEL does not bind PI(3)P, and that this lipid is not concentrated in the ER. We find that RxLR motifs cannot mediate export in P. falciparum. Parasites expressing a mutated version of KAHRP, with the PEXEL motif repositioned near the signal sequence, prevented PMV cleavage. This mutant possessed the putative PI(3)P-binding residues but is not exported. Reinstatement of PEXEL to its original location restores processing by PMV and export. These results challenge the PI(3) P hypothesis and provide evidence that PEXEL position is conserved for co-translational processing and export.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Boddey, Justin A.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
O'Neill, Matthew T.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Lopaticki, SashUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Carvalho, Teresa G.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hodder, Anthony N.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Nebl, ThomasUNSPECIFIEDorcid.org/0000-0003-4003-9340UNSPECIFIED
Wawra, StephanUNSPECIFIEDorcid.org/0000-0001-8555-1618UNSPECIFIED
van West, PieterUNSPECIFIEDorcid.org/0000-0002-0767-6017UNSPECIFIED
Ebrahimzadeh, ZeinabUNSPECIFIEDorcid.org/0000-0003-2972-4515UNSPECIFIED
Richard, DaveUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Flemming, SvenUNSPECIFIEDorcid.org/0000-0001-6461-5870UNSPECIFIED
Spielmann, TobiasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Przyborski, JudeUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Babon, Jeff J.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Cowman, Alan F.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-286588
DOI: 10.1038/ncomms10470
Journal or Publication Title: Nat. Commun.
Volume: 7
Date: 2016
Publisher: NATURE PUBLISHING GROUP
Place of Publication: LONDON
ISSN: 2041-1723
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
FALCIPARUM-INFECTED ERYTHROCYTES; PLASMODIUM-FALCIPARUM; HOST-CELL; MAURERS CLEFTS; PHYTOPHTHORA-INFESTANS; PLASMEPSIN-V; FYVE DOMAIN; ENDOPLASMIC-RETICULUM; EFFECTOR PROTEINS; TARGETING SIGNALMultiple languages
Multidisciplinary SciencesMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/28658

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