Universität zu Köln

Pannen, Derk, Fabisch, Maria, Gausling, Lisa and Schnetz, Karin ORCID: 0000-0003-4693-6202 (2016). Interaction of the RcsB Response Regulator with Auxiliary Transcription Regulators in Escherichia coli. J. Biol. Chem., 291 (5). S. 2357 - 2371. BETHESDA: AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC. ISSN 1083-351X

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Abstract

The Rcs phosphorelay is a two-component signal transduction system that is induced by cell envelope stress. RcsB, the response regulator of this signaling system, is a pleiotropic transcription regulator, which is involved in the control of various stress responses, cell division, motility, and biofilm formation. RcsB regulates transcription either as a homodimer or together with auxiliary regulators, such as RcsA, BglJ, and GadE in Escherichia coli. In this study, we show that RcsB in addition forms heterodimers with MatA (also known as EcpR) and with DctR. Our data suggest that the MatA-dependent transcription regulation is mediated by the MatA-RcsB heterodimer and is independent of RcsB phosphorylation. Furthermore, we analyzed the relevance of amino acid residues of the active quintet of conserved residues, and of surface-exposed residues for activity of RcsB. The data suggest that the activity of the phosphorylation-dependent dimers, such as RcsA-RcsB and RcsB-RcsB, is affected by mutation of residues in the vicinity of the phosphorylation site, suggesting that a phosphorylation-induced structural change modulates their activity. In contrast, the phosphorylation-independent heterodimers BglJ-RcsB and MatA-RcsB are affected by only very few mutations. Heterodimerization of RcsB with various auxiliary regulators and their differential dependence on phosphorylation add an additional level of control to the Rcs system that is operating at the output level.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Pannen, Derk UNSPECIFIED UNSPECIFIED UNSPECIFIED Fabisch, Maria UNSPECIFIED UNSPECIFIED UNSPECIFIED Gausling, Lisa UNSPECIFIED UNSPECIFIED UNSPECIFIED Schnetz, Karin UNSPECIFIED orcid.org/0000-0003-4693-6202 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-286908
DOI:	10.1074/jbc.M115.696815
Journal or Publication Title:	J. Biol. Chem.
Volume:	291
Number:	5
Page Range:	S. 2357 - 2371
Date:	2016
Publisher:	AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Place of Publication:	BETHESDA
ISSN:	1083-351X
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Institute for Genetics
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CAPSULAR POLYSACCHARIDE SYNTHESIS; H-NS; ACID RESISTANCE; ANTIBIOTIC-RESISTANCE; SIGNAL-TRANSDUCTION; ENVELOPE STRESS; COLANIC ACID; MAT FIMBRIAE; BGL OPERON; PHOSPHORELAY Multiple languages Biochemistry & Molecular Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/28690