Universität zu Köln

Dassler-Plenker, Juliane, Reiners, Katrin S., van den Boorn, Jasper G., Hansen, Hinrich P., Putschli, Bastian, Barnert, Sabine, Schuberth-Wagner, Christine, Schubert, Rolf, Tueting, Thomas, Hallek, Michael, Schlee, Martin, Hartmann, Gunther ORCID: 0000-0003-1021-2018, von Strandmann, Elke Pogge and Coch, Christoph (2016). RIG-I activation induces the release of extracellular vesicles with antitumor activity. OncoImmunology, 5 (10). PHILADELPHIA: TAYLOR & FRANCIS INC. ISSN 2162-402X

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Abstract

Activation of the innate immune receptor retinoic acid-inducible gene I (RIG-I) by its specific ligand 5-triphosphate-RNA (3pRNA) triggers antitumor immunity predominantly via NK cell activation and direct apoptosis induction in tumor cells. However, how NK cells are mobilized to attack the tumor cells remains elusive. Here, we show that RIG-I activation induced the secretion of extracellular vesicles (EVs) from melanoma cells, which by themselves revealed antitumor activity in vitro and in vivo. RIG-I-induced EVs from melanoma cells exhibited an increased expression of the NKp30-ligand (BAG6, BAT3) on their surface triggering NK cell-mediated lysis of melanoma cells via activation of the cytotoxicity NK cell-receptor NKp30. Moreover, systemic administration of RIG-I-induced melanoma-EVs showed a potent antitumor activity in a melanoma mouse model in vivo. In conclusion, our data establish a new RIG-I-dependent pathway leading to NK cell-mediated tumor cell killing.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Dassler-Plenker, Juliane UNSPECIFIED UNSPECIFIED UNSPECIFIED Reiners, Katrin S. UNSPECIFIED UNSPECIFIED UNSPECIFIED van den Boorn, Jasper G. UNSPECIFIED UNSPECIFIED UNSPECIFIED Hansen, Hinrich P. UNSPECIFIED UNSPECIFIED UNSPECIFIED Putschli, Bastian UNSPECIFIED UNSPECIFIED UNSPECIFIED Barnert, Sabine UNSPECIFIED UNSPECIFIED UNSPECIFIED Schuberth-Wagner, Christine UNSPECIFIED UNSPECIFIED UNSPECIFIED Schubert, Rolf UNSPECIFIED UNSPECIFIED UNSPECIFIED Tueting, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Hallek, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Schlee, Martin UNSPECIFIED UNSPECIFIED UNSPECIFIED Hartmann, Gunther UNSPECIFIED orcid.org/0000-0003-1021-2018 UNSPECIFIED von Strandmann, Elke Pogge UNSPECIFIED UNSPECIFIED UNSPECIFIED Coch, Christoph UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-288815
DOI:	10.1080/2162402X.2016.1219827
Journal or Publication Title:	OncoImmunology
Volume:	5
Number:	10
Date:	2016
Publisher:	TAYLOR & FRANCIS INC
Place of Publication:	PHILADELPHIA
ISSN:	2162-402X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CELL-DERIVED EXOSOMES; PROMOTE TUMOR-GROWTH; CANCER CELLS; MEMBRANE-VESICLES; DENDRITIC CELLS; NKP30 RECEPTOR; RNA; MICROVESICLES; INNATE; HSP70 Multiple languages Oncology; Immunology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/28881