Universität zu Köln

Brandt, Christian, Zander, Esther, Pfeifer, Yvonne, Braun, Sascha D., Ehricht, Ralf, Makarewicz, Oliwia and Pletz, Mathias W. (2016). Development of a rapid diagnostic assay based on magnetic bead purification of OXA-beta-lactamase mRNA. Future Microbiol., 11 (5). S. 617 - 630. LONDON: FUTURE MEDICINE LTD. ISSN 1746-0921

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Abstract

Aim: To countermeasure the global spread of beta-lactamases, we developed a rapid molecular test for the highly variable OXA-beta-lactamases that allows minimizing the time to effective treatment. Methods: OXA-mRNA was specifically enriched from total RNA using group-specific biotinylated DNA probes and streptavidin-coated magnetic beads. Phylogenetic OXA groups were distinguished by PCR product size. Results: This mRNA fishing method is highly sensitive, yielding specific results from as little as 1 ng total RNA. It enables discrimination of OXA-extended substrate spectrum beta-lactamases and carbapenemases and the semi-quantitative detection of highly expressed ISAba1-controlled variants. Conclusion: Targeting mRNA with specific probes on magnetic beads will allow for adaptation to automated systems, such as point-of-care diagnostics.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Brandt, Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED Zander, Esther UNSPECIFIED UNSPECIFIED UNSPECIFIED Pfeifer, Yvonne UNSPECIFIED UNSPECIFIED UNSPECIFIED Braun, Sascha D. UNSPECIFIED UNSPECIFIED UNSPECIFIED Ehricht, Ralf UNSPECIFIED UNSPECIFIED UNSPECIFIED Makarewicz, Oliwia UNSPECIFIED UNSPECIFIED UNSPECIFIED Pletz, Mathias W. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-289945
DOI:	10.2217/fmb.16.13
Journal or Publication Title:	Future Microbiol.
Volume:	11
Number:	5
Page Range:	S. 617 - 630
Date:	2016
Publisher:	FUTURE MEDICINE LTD
Place of Publication:	LONDON
ISSN:	1746-0921
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language KLEBSIELLA-PNEUMONIAE; ACINETOBACTER-BAUMANNII; ESCHERICHIA-COLI; GLOBAL SPREAD; INFECTIONS; RESISTANCE; ENTEROBACTERIACEAE; MORTALITY; IMPACT; CARBAPENEMS Multiple languages Microbiology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/28994