Universität zu Köln

Haehnle, Bastian, Lamla, Markus, Sparrer, Konstantin M. J., Gather, Malte C. ORCID: 0000-0002-4857-5562 and Kuehne, Alexander J. C. . Narrow Stimulated Resonance Raman Scattering and WGM Lasing in Small Conjugated Polymer Particles for Live Cell Tagging and Tracking. Adv. Opt. Mater.. WEINHEIM: WILEY-V C H VERLAG GMBH. ISSN 2195-1071

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Abstract

Conjugated polymer particles are brightly fluorescing and stable materials for live cell imaging. Combination of conjugated polymers with a whispering gallery mode (WGM) resonator allows laser emission from microscale particles. Once internalized by cells, the mode pattern of the laser emission can be used for tagging and tracking, as each laser spectrum represents a bar code to identify individual cells. However, currently these particle systems are limited by their large size, which might interfere with cellular functions. Here, stimulated resonance Raman scattering (SRRS) in small conjugated polymer microparticles is presented as a new method for generating narrow emission as an alternative to WGM-based laser emission. This opens up spectral range for multiplexing optical readout and multicolor imaging of live cells. The synthesis of monodisperse micrometer-sized poly(fluorene-co-divinylbenzene) particles is discussed and their WGM and SRRS emission are characterized. Finally, how these particles and their emission can be employed in live cell imaging and tagging is showcased.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Haehnle, Bastian UNSPECIFIED UNSPECIFIED UNSPECIFIED Lamla, Markus UNSPECIFIED UNSPECIFIED UNSPECIFIED Sparrer, Konstantin M. J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Gather, Malte C. UNSPECIFIED orcid.org/0000-0002-4857-5562 UNSPECIFIED Kuehne, Alexander J. C. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-308494
DOI:	10.1002/adom.202001553
Journal or Publication Title:	Adv. Opt. Mater.
Publisher:	WILEY-V C H VERLAG GMBH
Place of Publication:	WEINHEIM
ISSN:	2195-1071
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Chemistry > Institute of Physical Chemistry
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language AMPLIFIED SPONTANEOUS EMISSION; NANOPARTICLES Multiple languages Materials Science, Multidisciplinary; Optics Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/30849