Universität zu Köln

Patschan, Daniel, Schwarze, Katrin, Tampe, Bjorn, Becker, Jan Ulrich, Hakroush, Samy, Ritter, Oliver, Patschan, Susann and Mueller, Gerhard Anton (2020). Constitutive Atg5 overexpression in mouse bone marrow endothelial progenitor cells improves experimental acute kidney injury. BMC Nephrol., 21 (1). LONDON: BMC. ISSN 1471-2369

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Abstract

BackgroundEndothelial Progenitor Cells have been shown as effective tool in experimental AKI. Several pharmacological strategies for improving EPC-mediated AKI protection were identified in recent years. Aim of the current study was to analyze consequences of constitutive Atg5 activation in murine EPCs, utilized for AKI therapy.MethodsIschemic AKI was induced in male C57/Bl6N mice. Cultured murine EPCs were systemically injected post-ischemia, either natively or after Atg5 transfection (Adenovirus-based approach). Mice were analyzed 48h and 6weeks later.ResultsBoth, native and transfected EPCs (EPCsAtg5) improved persisting kidney dysfunction at week 6, such effects were more pronounced after injecting EPCsAtg5. While matrix deposition and mesenchymal transdifferentiation of endothelial cells remained unaffected by cell therapy, EPCs, particularly EPCsAtg5 completely prevented the post-ischemic loss of peritubular capillaries. The cells finally augmented the augophagocytic flux in endothelial cells.ConclusionsConstitutive Atg5 activation augments AKI-protective effects of murine EPCs. The exact clinical consequences need to be determined.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Patschan, Daniel UNSPECIFIED UNSPECIFIED UNSPECIFIED Schwarze, Katrin UNSPECIFIED UNSPECIFIED UNSPECIFIED Tampe, Bjorn UNSPECIFIED UNSPECIFIED UNSPECIFIED Becker, Jan Ulrich UNSPECIFIED UNSPECIFIED UNSPECIFIED Hakroush, Samy UNSPECIFIED UNSPECIFIED UNSPECIFIED Ritter, Oliver UNSPECIFIED UNSPECIFIED UNSPECIFIED Patschan, Susann UNSPECIFIED UNSPECIFIED UNSPECIFIED Mueller, Gerhard Anton UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-310882
DOI:	10.1186/s12882-020-02149-1
Journal or Publication Title:	BMC Nephrol.
Volume:	21
Number:	1
Date:	2020
Publisher:	BMC
Place of Publication:	LONDON
ISSN:	1471-2369
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MESENCHYMAL STEM-CELLS; AUTOPHAGY; MODULATION; AKI; THERAPIES; ISCHEMIA; PROTECT; EEOCS Multiple languages Urology & Nephrology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/31088