Knoop, Andre ORCID: 0000-0003-0040-8754, Planitz, Peter, Wuest, Bernhard and Thevis, Mario (2020). Analysis of cobalt for human sports drug testing purposes using ICP- and LC-ICP-MS. Drug Test. Anal., 12 (11-12). S. 1666 - 1673. HOBOKEN: WILEY. ISSN 1942-7611

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Abstract

Due to the current demands in the fight against manipulation of blood and blood components, commonly referred to as blood doping in sports drug testing, specific and sensitive detection methods enabling the detection of prohibited substances and methods of doping are required. Similar to illicit blood transfusions, erythropoiesis stimulating agents have been shown to be misused in sport, aiming at improving an athlete's aerobic capacity and endurance performance. Amongst other strategies, the administration of ionic cobalt (Co2+) can increase the number of erythrocytes by stimulating the endogenous erythropoietin (EPO) biosynthesis. Conversely, several organic Co-containing compounds such as cyanocobalamin (vitamin B12) are not prohibited in sports, and thus, an analytical differentiation of permitted and banned contributions to urinary Co-concentrations is desirable. An excretion study with daily applications of either 1 mg of CoCl2 or 1 mg of cyanocobalamin was conducted with 20 volunteers over a period of 14 consecutive days. Urine, plasma, and concentrated red blood cells were analyzed for their cobalt content. The samples were collected starting 7 days before the administration until 7 days after. Total Co concentrations were analyzed by using inductively coupled plasma mass spectrometry (ICP-MS), which yielded significantly elevated levels exclusively after inorganic cobalt intake. Furthermore, a liquid chromatography (LC)-ICP-MS approach was established and employed for the simultaneous determination of organically bound and inorganic cobalt by chromatographic separation within one single run. The analytical approach offers the option to further develop detection methods of illegal Co2+ supplementation in sport.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Knoop, AndreUNSPECIFIEDorcid.org/0000-0003-0040-8754UNSPECIFIED
Planitz, PeterUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wuest, BernhardUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Thevis, MarioUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-311330
DOI: 10.1002/dta.2962
Journal or Publication Title: Drug Test. Anal.
Volume: 12
Number: 11-12
Page Range: S. 1666 - 1673
Date: 2020
Publisher: WILEY
Place of Publication: HOBOKEN
ISSN: 1942-7611
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
PLASMA; MASS; ERYTHROPOIESIS; EXPOSURE; CHLORIDE; URINEMultiple languages
Biochemical Research Methods; Chemistry, Analytical; Pharmacology & PharmacyMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/31133

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