Universität zu Köln

Xanthopoulou, Kyriaki, Urrutikoetxea-Gutierrez, Mikel ORCID: 0000-0002-4388-0189, Vidal-Garcia, Matxalen, Diaz de Tuesta del Arco, Jose-Luis, Sanchez-Urtaza, Sandra, Wille, Julia, Seifert, Harald, Higgins, Paul G. ORCID: 0000-0001-8677-9454 and Gallego, Lucia (2020). First Report of New Delhi Metallo-beta-Lactamase-6 (NDM-6) in a Clinical Acinetobacter baumannii Isolate From Northern Spain. Front. Microbiol., 11. LAUSANNE: FRONTIERS MEDIA SA. ISSN 1664-302X

Full text not available from this repository.

Abstract

The objective of this study was the phenotypic and genotypic characterization of a carbapenem resistant Acinetobacter baumannii (CRAB) isolate. The isolate, recovered in Northern Spain in 2019, was identified by MALDI-TOF to the species level. Antimicrobial susceptibility testing was performed using the Phoenix BD NMIC-502 Panel, E-test, and broth microdilution methods. The presence of a metallo-beta-lactamase (MBL) was verified by PCR and immunochromatographic assays. The genetic location of the MBL was confirmed using S1-pulsed-field gel electrophoresis (S1-PFGE) followed by Southern blot hybridization. Whole genome sequencing (WGS) was completed using the Miseq and MinION platforms, followed by core-genome MLST (cgMLST) and seven-locus MLST analysis. The CRAB was assigned ST85 (Pasteur scheme) and ST957 (Oxford scheme) representing international clone (IC) 9 and harbored the intrinsic beta-lactamase OXA-94 with ISAba1 upstream of it, and the MBL bla(NDM-6). Hybridization experiments revealed that the bla(NDM-6) was encoded on the chromosome. Using WGS the bla(NDM-6) environment could be identified arranged in the following order: ISAba14, aphA6, ISAba125, bla(NDM-6), ble(MBL), trpF, dsbC, cutA, and ISAba14. Downstream, a 10,462 bp duplication was identified, including a second copy of bla(NDM-6) in the following genetic composition: ISAba125, bla(NDM-6), ble(MBL), trpF, dsbC, cutA, and ISAba14. To our knowledge, this is the first description of bla(NDM-6) in A. baumannii. The MBL was present in two copies in the chromosome in a new genetic environment associated with IS elements highlighting the contribution of mobile genetic elements in the dissemination of this gene.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Xanthopoulou, Kyriaki UNSPECIFIED UNSPECIFIED UNSPECIFIED Urrutikoetxea-Gutierrez, Mikel UNSPECIFIED orcid.org/0000-0002-4388-0189 UNSPECIFIED Vidal-Garcia, Matxalen UNSPECIFIED UNSPECIFIED UNSPECIFIED Diaz de Tuesta del Arco, Jose-Luis UNSPECIFIED UNSPECIFIED UNSPECIFIED Sanchez-Urtaza, Sandra UNSPECIFIED UNSPECIFIED UNSPECIFIED Wille, Julia UNSPECIFIED UNSPECIFIED UNSPECIFIED Seifert, Harald UNSPECIFIED UNSPECIFIED UNSPECIFIED Higgins, Paul G. UNSPECIFIED orcid.org/0000-0001-8677-9454 UNSPECIFIED Gallego, Lucia UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-311697
DOI:	10.3389/fmicb.2020.589253
Journal or Publication Title:	Front. Microbiol.
Volume:	11
Date:	2020
Publisher:	FRONTIERS MEDIA SA
Place of Publication:	LAUSANNE
ISSN:	1664-302X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PREVALENT OXA CARBAPENEMASES; MOLECULAR CHARACTERIZATION; RESISTANCE; ENTEROBACTERIACEAE; IDENTIFICATION; BLA(NDM-1); PCR Multiple languages Microbiology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/31169