Universität zu Köln

Henrich, Birgit, Hammerlage, Stephanie, Scharf, Sebastian, Haberhausen, Diana, Fuernkranz, Ursula, Kohrer, Karl, Peitzmann, Lena, Fiori, Pier Luigi ORCID: 0000-0001-6190-612X, Spergser, Joachim, Pfeffer, Klaus and Dilthey, Alexander T. (2020). Characterisation of mobile genetic elements in Mycoplasma hominis with the description of ICEHo-II, a variant mycoplasma integrative and conjugative element. Mob. DNA, 11 (1). LONDON: BMC. ISSN 1759-8753

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Abstract

Background Mobile genetic elements are found in genomes throughout the microbial world, mediating genome plasticity and important prokaryotic phenotypes. Even the cell wall-less mycoplasmas, which are known to harbour a minimal set of genes, seem to accumulate mobile genetic elements. In Mycoplasma hominis, a facultative pathogen of the human urogenital tract and an inherently very heterogeneous species, four different MGE-classes had been detected until now: insertion sequence ISMhom-1, prophage MHoV-1, a tetracycline resistance mediating transposon, and ICEHo, a species-specific variant of a mycoplasma integrative and conjugative element encoding a T4SS secretion system (termed MICE). Results To characterize the prevalence of these MGEs, genomes of 23 M. hominis isolates were assembled using whole genome sequencing and bioinformatically analysed for the presence of mobile genetic elements. In addition to the previously described MGEs, a new ICEHo variant was found, which we designate ICEHo-II. Of 15 ICEHo-II genes, five are common MICE genes; eight are unique to ICEHo-II; and two represent a duplication of a gene also present in ICEHo-I. In 150 M. hominis isolates and based on a screening PCR, prevalence of ICEHo-I was 40.7%; of ICEHo-II, 28.7%; and of both elements, 15.3%. Activity of ICEHo-I and -II was demonstrated by detection of circularized extrachromosomal forms of the elements through PCR and subsequent Sanger sequencing. Conclusions Nanopore sequencing enabled the identification of mobile genetic elements and of ICEHo-II, a novel MICE element of M. hominis, whose phenotypic impact and potential impact on pathogenicity can now be elucidated.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Henrich, Birgit UNSPECIFIED UNSPECIFIED UNSPECIFIED Hammerlage, Stephanie UNSPECIFIED UNSPECIFIED UNSPECIFIED Scharf, Sebastian UNSPECIFIED UNSPECIFIED UNSPECIFIED Haberhausen, Diana UNSPECIFIED UNSPECIFIED UNSPECIFIED Fuernkranz, Ursula UNSPECIFIED UNSPECIFIED UNSPECIFIED Kohrer, Karl UNSPECIFIED UNSPECIFIED UNSPECIFIED Peitzmann, Lena UNSPECIFIED UNSPECIFIED UNSPECIFIED Fiori, Pier Luigi UNSPECIFIED orcid.org/0000-0001-6190-612X UNSPECIFIED Spergser, Joachim UNSPECIFIED UNSPECIFIED UNSPECIFIED Pfeffer, Klaus UNSPECIFIED UNSPECIFIED UNSPECIFIED Dilthey, Alexander T. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-311947
DOI:	10.1186/s13100-020-00225-9
Journal or Publication Title:	Mob. DNA
Volume:	11
Number:	1
Date:	2020
Publisher:	BMC
Place of Publication:	LONDON
ISSN:	1759-8753
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SEQUENCE; SEARCH Multiple languages Genetics & Heredity Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/31194