Universität zu Köln

Diaz-Gomez, Jorge L., Neundorf, Ines ORCID: 0000-0001-6450-3991, Lopez-Castillo, Laura-Margarita, Castorena-Torres, Fabiola, Serna-Saldivar, Sergio O. and Garcia-Lara, Silverio (2020). In Silico Analysis and In Vitro Characterization of the Bioactive Profile of Three Novel Peptides Identified from 19 kDa alpha-Zein Sequences of Maize. Molecules, 25 (22). BASEL: MDPI. ISSN 1420-3049

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Abstract

In this study, we characterized three novel peptides derived from the 19 kDa alpha-zein, and determined their bioactive profile in vitro and developed a structural model in silico. The peptides, 19ZP1, 19ZP2 and 19ZP3, formed alpha-helical structures and had positive and negative electrostatic potential surfaces (range of -1 to +1). According to the in silico algorithms, the peptides displayed low probabilities for cytotoxicity (<= 0.05%), cell penetration (10-33%) and antioxidant activities (9-12.5%). Instead, they displayed a 40% probability for angiotensin-converting enzyme (ACE) inhibitory activity. For in vitro characterization, peptides were synthesized by solid phase synthesis and tested accordingly. We assumed alpha-helical structures for 19ZP1 and 19ZP2 under hydrophobic conditions. The peptides displayed antioxidant activity and ACE-inhibitory activity, with 19ZP1 being the most active. Our results highlight that the 19 kDa alpha-zein sequences could be explored as a source of bioactive peptides, and indicate that in silico approaches are useful to predict peptide bioactivities, but more structural analysis is necessary to obtain more accurate data.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Diaz-Gomez, Jorge L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Neundorf, Ines UNSPECIFIED orcid.org/0000-0001-6450-3991 UNSPECIFIED Lopez-Castillo, Laura-Margarita UNSPECIFIED UNSPECIFIED UNSPECIFIED Castorena-Torres, Fabiola UNSPECIFIED UNSPECIFIED UNSPECIFIED Serna-Saldivar, Sergio O. UNSPECIFIED UNSPECIFIED UNSPECIFIED Garcia-Lara, Silverio UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-312924
DOI:	10.3390/molecules25225405
Journal or Publication Title:	Molecules
Volume:	25
Number:	22
Date:	2020
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	1420-3049
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Chemistry > Institute of Biochemistry
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CELL-PENETRATING PEPTIDES; CONVERTING ENZYME-INHIBITORS; QUALITY PROTEIN MAIZE; ANTIOXIDANT PEPTIDES; CORN PEPTIDES; FUNCTIONAL-CHARACTERIZATION; ANTIHYPERTENSIVE PEPTIDES; MASS SPECTROMETRY; HYDROLYSATE; FRACTIONS Multiple languages Biochemistry & Molecular Biology; Chemistry, Multidisciplinary Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/31292