Universität zu Köln

Koeditz, Barbara, Fries, Jochen W. U., Goebel, Heike, Paffenholz, Pia, Richter, Konstantin, Heidenreich, Axel and Von Brandenstein, Melanie (2020). Mxi-2 Dependent Regulation of p53 in Prostate Cancer. Anticancer Res., 40 (10). S. 5539 - 5545. ATHENS: INT INST ANTICANCER RESEARCH. ISSN 1791-7530

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Abstract

Background/Aim: Endothelin-1 (ET-1) is overexpressed in many types of cancer, inhibiting the release of the microRNA 15a (miR-15a) and inducing the production of Mxi-2. Our aim was to identify a molecular complex regulating p53 activity in prostate cancer (PCa). Materials and Methods: DU145 cells were treated with ET-1, MAPK p38 inhibitor, Endothelin A receptor inhibitor (ETAR inhibitor) and Endothelin B receptor inhibitor (ETBR inhibitor). Extracts were analysed using Western Blot, immunoprecipitation and qRT-PCR. Furthermore, prostate cancer patient samples were analysed using qRT-PCR and ELISA. Results: The hypothesised molecular complex was identified, with miR-15a, microRNA 1285 (miR-1285) and Mxi-2 levels up-regulated in patients in relation to increasing aggressiveness of PCa. Conclusion: A complex composed of Argonaut 2 (Ago2)/Mxi-2/miR-1285 is involved in PCa. The expression of Mxi-2 correlates with increasing PCa aggressiveness and might be used as a noninvasive marker for the diagnosis and progression of PCa.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Koeditz, Barbara UNSPECIFIED UNSPECIFIED UNSPECIFIED Fries, Jochen W. U. UNSPECIFIED UNSPECIFIED UNSPECIFIED Goebel, Heike UNSPECIFIED UNSPECIFIED UNSPECIFIED Paffenholz, Pia UNSPECIFIED UNSPECIFIED UNSPECIFIED Richter, Konstantin UNSPECIFIED UNSPECIFIED UNSPECIFIED Heidenreich, Axel UNSPECIFIED UNSPECIFIED UNSPECIFIED Von Brandenstein, Melanie UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-316605
DOI:	10.21873/anticanres.14566
Journal or Publication Title:	Anticancer Res.
Volume:	40
Number:	10
Page Range:	S. 5539 - 5545
Date:	2020
Publisher:	INT INST ANTICANCER RESEARCH
Place of Publication:	ATHENS
ISSN:	1791-7530
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language KINASE-C-ALPHA; PROTEIN; ENDOTHELIN-1 Multiple languages Oncology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/31660