Universität zu Köln

Kahle, Nikolai, Sheerin, David J., Fischbach, Patrick, Koch, Leonie-Alexa, Schwenk, Philipp, Lambert, Dorothee, Rodriguez, Ryan, Kerner, Konstantin, Hoecker, Ute ORCID: 0000-0002-5636-9777, Zurbriggen, Matias D. ORCID: 0000-0002-3523-2907 and Hiltbrunner, Andreas (2020). COLD REGULATED 27 and 28 are targets of CONSTITUTIVELY PHOTOMORPHOGENIC 1 and negatively affect phytochrome B signalling. Plant J., 104 (4). S. 1038 - 1054. HOBOKEN: WILEY. ISSN 1365-313X

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Abstract

Phytochromes are red/far-red light receptors in plants involved in the regulation of growth and development. Phytochromes can sense the light environment and contribute to measuring day length; thereby, they allow plants to respond and adapt to changes in the ambient environment. Two well-characterized signalling pathways act downstream of phytochromes and link light perception to the regulation of gene expression. The CONSTITUTIVELY PHOTOMORPHOGENIC 1/SUPPRESSOR OF PHYA-105 (COP1/SPA) E3 ubiquitin ligase complex and the PHYTOCHROME INTERACTING FACTORs (PIFs) are key components of these pathways and repress light responses in the dark. In light-grown seedlings, phytochromes inhibit COP1/SPA and PIF activity and thereby promote light signalling. In a yeast-two-hybrid screen for proteins binding to light-activated phytochromes, we identified COLD-REGULATED GENE 27 (COR27). COR27 and its homologue COR28 bind to phyA and phyB, the two primary phytochromes in seed plants. COR27 and COR28 have been described previously with regard to a function in the regulation of freezing tolerance, flowering and the circadian clock. Here, we show that COR27 and COR28 repress early seedling development in blue, far-red and in particular red light. COR27 and COR28 contain a conserved Val-Pro (VP)-peptide motif, which mediates binding to the COP1/SPA complex. COR27 and COR28 are targeted for degradation by COP1/SPA and mutant versions with a VP to AA amino acid substitution in the VP-peptide motif are stabilized. Overall, our data suggest that COR27 and COR28 accumulate in light but act as negative regulators of light signalling during early seedling development, thereby preventing an exaggerated response to light.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Kahle, Nikolai UNSPECIFIED UNSPECIFIED UNSPECIFIED Sheerin, David J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Fischbach, Patrick UNSPECIFIED UNSPECIFIED UNSPECIFIED Koch, Leonie-Alexa UNSPECIFIED UNSPECIFIED UNSPECIFIED Schwenk, Philipp UNSPECIFIED UNSPECIFIED UNSPECIFIED Lambert, Dorothee UNSPECIFIED UNSPECIFIED UNSPECIFIED Rodriguez, Ryan UNSPECIFIED UNSPECIFIED UNSPECIFIED Kerner, Konstantin UNSPECIFIED UNSPECIFIED UNSPECIFIED Hoecker, Ute UNSPECIFIED orcid.org/0000-0002-5636-9777 UNSPECIFIED Zurbriggen, Matias D. UNSPECIFIED orcid.org/0000-0002-3523-2907 UNSPECIFIED Hiltbrunner, Andreas UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-318250
DOI:	10.1111/tpj.14979
Journal or Publication Title:	Plant J.
Volume:	104
Number:	4
Page Range:	S. 1038 - 1054
Date:	2020
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1365-313X
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language COP1 E3 LIGASE; ARABIDOPSIS PHYTOCHROME; INTERACTING FACTOR; CIRCADIAN CLOCK; GENE-EXPRESSION; FLORAL-DIP; LIGHT; PROTEINS; SPA; DEGRADATION Multiple languages Plant Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/31825