Universität zu Köln

Higgins, Paul G. ORCID: 0000-0001-8677-9454, Hagen, Ralf Matthias, Podbielski, Andreas, Frickmann, Hagen and Warnke, Philipp (2020). Molecular Epidemiology of Carbapenem-Resistant Acinetobacter baumannii Isolated from War-Injured Patients from the Eastern Ukraine. Antibiotics-Basel, 9 (9). BASEL: MDPI. ISSN 2079-6382

Full text not available from this repository.

Abstract

Recently, a total of 32 carbapenem- and fluoroquinolone-resistant Acinetobacter baumannii (Ab) isolates was isolated from war-injured patients who were treated at German Bundeswehr Hospitals, and preliminarily typed by DiversiLab repetitive elements sequence-based (rep-) PCR. Core genome-based sequence typing was also used to provide more detailed epidemiological information. From the clusters observed by rep-PCR, selected Ab strains were subjected to Next Generation Sequencing (NGS) in order to compare them with international outbreak-associated Ab strains and to identify MLST (multi-locus sequence type) lineages, as well as to identify known resistance genes. Accordingly, NGS indicated higher diversity than rep-PCR, but also confirmed likely transmission events. The identified acquired carbapenem-resistant genes comprised bla(OXA-23), bla(OXA-72) and bla(GES-12), as well as various other intrinsic and acquired resistance-associated genetic elements. All isolates clustered with the previously identified international clonal lineages IC1, IC2, IC6 and IC7, with corresponding Pasteur sequence types ST1, ST2, ST78 and ST25, respectively. In conclusion, the assessment confirmed a broad spectrum of resistance-associated genes in Ab isolated from war-injured patients from the Eastern Ukraine, and provided the first insights into locally abundant clonal lineages.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Higgins, Paul G. UNSPECIFIED orcid.org/0000-0001-8677-9454 UNSPECIFIED Hagen, Ralf Matthias UNSPECIFIED UNSPECIFIED UNSPECIFIED Podbielski, Andreas UNSPECIFIED UNSPECIFIED UNSPECIFIED Frickmann, Hagen UNSPECIFIED UNSPECIFIED UNSPECIFIED Warnke, Philipp UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-320186
DOI:	10.3390/antibiotics9090579
Journal or Publication Title:	Antibiotics-Basel
Volume:	9
Number:	9
Date:	2020
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	2079-6382
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SEQUENCE TYPING SCHEME; CALCOACETICUS COMPLEX; WOUND INFECTIONS; MILITARY; BACTERIA; IRAQ; PERSONNEL; RECOVERY; AFGHANISTAN Multiple languages Infectious Diseases; Pharmacology & Pharmacy Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/32018