Universität zu Köln

Boyen, Jens ORCID: 0000-0001-5005-7724, Fink, Patrick ORCID: 0000-0002-5927-8977, Mensens, Christoph, Habluetzel, Pascal, I and De Troche, Marleen (2020). Fatty acid bioconversion in harpacticoid copepods in a changing environment: a transcriptomic approach. Philos. Trans. R. Soc. B-Biol. Sci., 375 (1804). LONDON: ROYAL SOC. ISSN 1471-2970

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Abstract

By 2100, global warming is predicted to significantly reduce the capacity of marine primary producers for long-chain polyunsaturated fatty acid (LC-PUFA) synthesis. Primary consumers such as harpacticoid copepods (Crustacea) might mitigate the resulting adverse effects on the food web by increased LC-PUFA bioconversion. Here, we present a high-quality de novo transcriptome assembly of the copepodPlatychelipus littoralis, exposed to changes in both temperature (+3 degrees C) and dietary LC-PUFA availability. Using this transcriptome, we detected multiple transcripts putatively coding for LC-PUFA-bioconverting front-end fatty acid (FA) desaturases and elongases, and performed phylogenetic analyses to identify their relationship with sequences of other (crustacean) taxa. While temperature affected the absolute FA concentrations in copepods, LC-PUFA levels remained unaltered even when copepods were fed an LC-PUFA-deficient diet. While this suggests plasticity of LC-PUFA bioconversion withinP. littoralis, none of the putative front-end desaturase or elongase transcripts was differentially expressed under the applied treatments. Nevertheless, the transcriptome presented here provides a sound basis for future ecophysiological research on harpacticoid copepods. This article is part of the theme issue 'The next horizons for lipids as 'trophic biomarkers': evidence and significance of consumer modification of dietary fatty acids'.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Boyen, Jens UNSPECIFIED orcid.org/0000-0001-5005-7724 UNSPECIFIED Fink, Patrick UNSPECIFIED orcid.org/0000-0002-5927-8977 UNSPECIFIED Mensens, Christoph UNSPECIFIED UNSPECIFIED UNSPECIFIED Habluetzel, Pascal, I UNSPECIFIED UNSPECIFIED UNSPECIFIED De Troche, Marleen UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-324060
DOI:	10.1098/rstb.2019.0645
Journal or Publication Title:	Philos. Trans. R. Soc. B-Biol. Sci.
Volume:	375
Number:	1804
Date:	2020
Publisher:	ROYAL SOC
Place of Publication:	LONDON
ISSN:	1471-2970
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language NUTRITIONAL REGULATION; TISSUE DISTRIBUTION; LIPID-COMPOSITION; MUD CRAB; GENE; TEMPERATURE; EXPRESSION; CLONING; FOOD; IDENTIFICATION Multiple languages Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/32406