Universität zu Köln

Harvey, Sarah, Kumari, Priyanka, Lapin, Dmitry ORCID: 0000-0001-9591-3950, Griebel, Thomas ORCID: 0000-0002-3600-6493, Hickman, Richard ORCID: 0000-0002-4542-7740, Guo, Wenbin ORCID: 0000-0002-1829-6044, Zhang, Runxuan, Parker, Jane E., Beynon, Jim, Denby, Katherine ORCID: 0000-0002-7857-6814 and Steinbrenner, Jens (2020). Downy Mildew effector HaRxL21 interacts with the transcriptional repressor TOPLESS to promote pathogen susceptibility. PLoS Pathog., 16 (8). SAN FRANCISCO: PUBLIC LIBRARY SCIENCE. ISSN 1553-7374

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Abstract

Author summary Disease in plants depends on the outcome of interaction between the pathogen and the host plant. Pathogens use effector proteins to aid colonization by suppression of the host immune system. Identification of effector targets can pinpoint key components of plant defence in addition to aiding our understanding of how pathogens manipulate their hosts. Here, we see a downy mildew effector mimicking a plant transcriptional repression motif and interacting with a transcriptional co-repressor in order to manipulate the plant immunity transcriptional response and susceptibility to both a biotrophic and a necrotrophic pathogen. Hyaloperonospora arabidopsidis(Hpa) is an oomycete pathogen causing Arabidopsis downy mildew. Effector proteins secreted from the pathogen into the plant play key roles in promoting infection by suppressing plant immunity and manipulating the host to the pathogen's advantage. One class of oomycete effectors share a conserved 'RxLR' motif critical for their translocation into the host cell. Here we characterize the interaction between an RxLR effector, HaRxL21 (RxL21), and the Arabidopsis transcriptional co-repressor Topless (TPL). We establish that RxL21 and TPL interact via an EAR motif at the C-terminus of the effector, mimicking the host plant mechanism for recruiting TPL to sites of transcriptional repression. We show that this motif, and hence interaction with TPL, is necessary for the virulence function of the effector. Furthermore, we provide evidence that RxL21 uses the interaction with TPL, and its close relative TPL-related 1, to repress plant immunity and enhance host susceptibility to both biotrophic and necrotrophic pathogens.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Harvey, Sarah UNSPECIFIED UNSPECIFIED UNSPECIFIED Kumari, Priyanka UNSPECIFIED UNSPECIFIED UNSPECIFIED Lapin, Dmitry UNSPECIFIED orcid.org/0000-0001-9591-3950 UNSPECIFIED Griebel, Thomas UNSPECIFIED orcid.org/0000-0002-3600-6493 UNSPECIFIED Hickman, Richard UNSPECIFIED orcid.org/0000-0002-4542-7740 UNSPECIFIED Guo, Wenbin UNSPECIFIED orcid.org/0000-0002-1829-6044 UNSPECIFIED Zhang, Runxuan UNSPECIFIED UNSPECIFIED UNSPECIFIED Parker, Jane E. UNSPECIFIED UNSPECIFIED UNSPECIFIED Beynon, Jim UNSPECIFIED UNSPECIFIED UNSPECIFIED Denby, Katherine UNSPECIFIED orcid.org/0000-0002-7857-6814 UNSPECIFIED Steinbrenner, Jens UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-324712
DOI:	10.1371/journal.ppat.1008835
Journal or Publication Title:	PLoS Pathog.
Volume:	16
Number:	8
Date:	2020
Publisher:	PUBLIC LIBRARY SCIENCE
Place of Publication:	SAN FRANCISCO
ISSN:	1553-7374
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language RECEPTOR-LIKE KINASE; INNATE IMMUNITY; ARABIDOPSIS-THALIANA; TRIGGERED IMMUNITY; CELL-SURFACE; EAR MOTIF; PROTEIN; GENE; RESISTANCE; PLANTS Multiple languages Microbiology; Parasitology; Virology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/32471