Kontopoulos, Ioannis ORCID: 0000-0001-5591-8917, Penkman, Kirsty, Mullin, Victoria E., Winkelbach, Laura, Unterlaender, Martina, Scheu, Amelie, Kreutzer, Susanne ORCID: 0000-0001-6286-534X, Hansen, Henrik B., Margaryan, Ashot ORCID: 0000-0002-2576-2429, Teasdale, Matthew D., Gehlen, Birgit, Street, Martin, Lynnerup, Niels, Liritzis, Ioannis, Sampson, Adamantios, Papageorgopoulou, Christina, Allentoft, Morten E., Burger, Joachim, Bradley, Daniel G. and Collins, Matthew J. (2020). Screening archaeological bone for palaeogenetic and palaeoproteomic studies. PLoS One, 15 (6). SAN FRANCISCO: PUBLIC LIBRARY SCIENCE. ISSN 1932-6203

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Abstract

The recovery and analysis of ancient DNA and protein from archaeological bone is time-consuming and expensive to carry out, while it involves the partial or complete destruction of valuable or rare specimens. The fields of palaeogenetic and palaeoproteomic research would benefit greatly from techniques that can assess the molecular quality prior to sampling. To be relevant, such screening methods should be effective, minimally-destructive, and rapid. This study reports results based on spectroscopic (Fourier-transform infrared spectroscopy in attenuated total reflectance [FTIR-ATR]; n = 266), palaeoproteomic (collagen content; n = 226), and palaeogenetic (endogenous DNA content; n = 88) techniques. We establish thresholds for three different FTIR indices, a) the infrared splitting factor [IRSF] that assesses relative changes in bioapatite crystals' size and homogeneity; b) the carbonate-to-phosphate [C/P] ratio as a relative measure of carbonate content in bioapatite crystals; and c) the amide-to-phosphate ratio [Am/P] for assessing the relative organic content preserved in bone. These thresholds are both extremely reliable and easy to apply for the successful and rapid distinction between well- and poorly-preserved specimens. This is a milestone for choosing appropriate samples prior to genomic and collagen analyses, with important implications for biomolecular archaeology and palaeontology.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Kontopoulos, IoannisUNSPECIFIEDorcid.org/0000-0001-5591-8917UNSPECIFIED
Penkman, KirstyUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Mullin, Victoria E.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Winkelbach, LauraUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Unterlaender, MartinaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Scheu, AmelieUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kreutzer, SusanneUNSPECIFIEDorcid.org/0000-0001-6286-534XUNSPECIFIED
Hansen, Henrik B.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Margaryan, AshotUNSPECIFIEDorcid.org/0000-0002-2576-2429UNSPECIFIED
Teasdale, Matthew D.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gehlen, BirgitUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Street, MartinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Lynnerup, NielsUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Liritzis, IoannisUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Sampson, AdamantiosUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Papageorgopoulou, ChristinaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Allentoft, Morten E.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Burger, JoachimUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bradley, Daniel G.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Collins, Matthew J.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-329167
DOI: 10.1371/journal.pone.0235146
Journal or Publication Title: PLoS One
Volume: 15
Number: 6
Date: 2020
Publisher: PUBLIC LIBRARY SCIENCE
Place of Publication: SAN FRANCISCO
ISSN: 1932-6203
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
AMINO-ACID RACEMIZATION; ANCIENT DNA; PRESERVATION; COLLAGEN; DIAGENESIS; IDENTIFICATION; DEGRADATION; CARBON; SITES; DECAYMultiple languages
Multidisciplinary SciencesMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/32916

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