Universität zu Köln

Mueller-Schuessele, Stefanie J., Wang, Ren, Guetle, Desiree D., Romer, Jill, Rodriguez-Franco, Marta, Scholz, Martin, Buchert, Felix ORCID: 0000-0001-6704-6634, Lueth, Volker M., Kopriva, Stanislav ORCID: 0000-0002-7416-6551, Doermann, Peter, Schwarzlaender, Markus, Reski, Ralf, Hippler, Michael and Meyer, Andreas J. (2020). Chloroplasts require glutathione reductase to balance reactive oxygen species and maintain efficient photosynthesis. Plant J., 103 (3). S. 1140 - 1155. HOBOKEN: WILEY. ISSN 1365-313X

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Abstract

Thiol-based redox-regulation is vital for coordinating chloroplast functions depending on illumination and has been throroughly investigated for thioredoxin-dependent processes. In parallel, glutathione reductase (GR) maintains a highly reduced glutathione pool, enabling glutathione-mediated redox buffering. Yet, how the redox cascades of the thioredoxin and glutathione redox machineries integrate metabolic regulation and detoxification of reactive oxygen species remains largely unresolved because null mutants of plastid/mitochondrial GR are embryo-lethal in Arabidopsis thaliana. To investigate whether maintaining a highly reducing stromal glutathione redox potential (E-GSH) via GR is necessary for functional photosynthesis and plant growth, we created knockout lines of the homologous enzyme in the model moss Physcomitrella patens. In these viable mutant lines, we found decreasing photosynthetic performance and plant growth with increasing light intensities, whereas ascorbate and zeaxanthin/antheraxanthin levels were elevated. By in vivo monitoring stromal E-GSH dynamics, we show that stromal E-GSH is highly reducing in wild-type and clearly responsive to light, whereas an absence of GR leads to a partial glutathione oxidation, which is not rescued by light. By metabolic labelling, we reveal changing protein abundances in the GR knockout plants, pinpointing the adjustment of chloroplast proteostasis and the induction of plastid protein repair and degradation machineries. Our results indicate that the plastid thioredoxin system is not a functional backup for the plastid glutathione redox systems, whereas GR plays a critical role in maintaining efficient photosynthesis.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Mueller-Schuessele, Stefanie J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Wang, Ren UNSPECIFIED UNSPECIFIED UNSPECIFIED Guetle, Desiree D. UNSPECIFIED UNSPECIFIED UNSPECIFIED Romer, Jill UNSPECIFIED UNSPECIFIED UNSPECIFIED Rodriguez-Franco, Marta UNSPECIFIED UNSPECIFIED UNSPECIFIED Scholz, Martin UNSPECIFIED UNSPECIFIED UNSPECIFIED Buchert, Felix UNSPECIFIED orcid.org/0000-0001-6704-6634 UNSPECIFIED Lueth, Volker M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Kopriva, Stanislav UNSPECIFIED orcid.org/0000-0002-7416-6551 UNSPECIFIED Doermann, Peter UNSPECIFIED UNSPECIFIED UNSPECIFIED Schwarzlaender, Markus UNSPECIFIED UNSPECIFIED UNSPECIFIED Reski, Ralf UNSPECIFIED UNSPECIFIED UNSPECIFIED Hippler, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Meyer, Andreas J. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-333078
DOI:	10.1111/tpj.14791
Journal or Publication Title:	Plant J.
Volume:	103
Number:	3
Page Range:	S. 1140 - 1155
Date:	2020
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1365-313X
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language REDOX REGULATION; PHYSCOMITRELLA-PATENS; MITOCHONDRIAL ENERGY; XANTHOPHYLL CYCLE; IN-VIVO; ASCORBATE; THIOREDOXIN; PLANTS; MOSS; PHOTOINHIBITION Multiple languages Plant Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/33307