Universität zu Köln

Cerezales, Monica ORCID: 0000-0001-6705-006X, Xanthopoulou, Kyriaki ORCID: 0000-0001-8591-8184, Wille, Julia, Krut, Oleg, Seifert, Harald, Gallego, Lucia and Higgins, Paul G. ORCID: 0000-0001-8677-9454 (2020). Mobile Genetic Elements Harboring Antibiotic Resistance Determinants in Acinetobacter baumannii Isolates From Bolivia. Front. Microbiol., 11. LAUSANNE: FRONTIERS MEDIA SA. ISSN 1664-302X

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Abstract

Using a combination of short- and long-read DNA sequencing, we have investigated the location of antibiotic resistance genes and characterized mobile genetic elements (MGEs) in three clinical multi-drug resistant Acinetobacter baumannii. The isolates, collected in Bolivia, clustered separately with three different international clonal lineages. We found a diverse array of transposons, plasmids and resistance islands related to different insertion sequence (IS) elements, which were located in both the chromosome and in plasmids, which conferred resistance to multiple antimicrobials, including carbapenems. Carbapenem resistance might be caused by a Tn2008 carrying the bla(OXA-23) gene. Some plasmids were shared between the isolates. Larger plasmids were less conserved than smaller ones and they shared some homologous regions, while others were more diverse, suggesting that these big plasmids are more plastic than the smaller ones. The genetic basis of antimicrobial resistance in Bolivia has not been deeply studied until now, and the mobilome of these A. baumannii isolates, combined with their multi-drug resistant phenotype, mirror the transfer and prevalence of MGEs contributing to the spread of antibiotic resistance worldwide and require special attention. These findings could be useful to understand the antimicrobial resistance genetics of A. baumannii in Bolivia and the difficulty in tackling these infections.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Cerezales, Monica UNSPECIFIED orcid.org/0000-0001-6705-006X UNSPECIFIED Xanthopoulou, Kyriaki UNSPECIFIED orcid.org/0000-0001-8591-8184 UNSPECIFIED Wille, Julia UNSPECIFIED UNSPECIFIED UNSPECIFIED Krut, Oleg UNSPECIFIED UNSPECIFIED UNSPECIFIED Seifert, Harald UNSPECIFIED UNSPECIFIED UNSPECIFIED Gallego, Lucia UNSPECIFIED UNSPECIFIED UNSPECIFIED Higgins, Paul G. UNSPECIFIED orcid.org/0000-0001-8677-9454 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-333845
DOI:	10.3389/fmicb.2020.00919
Journal or Publication Title:	Front. Microbiol.
Volume:	11
Date:	2020
Publisher:	FRONTIERS MEDIA SA
Place of Publication:	LAUSANNE
ISSN:	1664-302X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SPREAD; EMERGENCE; PLASMIDS Multiple languages Microbiology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/33384