Universität zu Köln

Lueke, Jan Niklas, Neumaier, Felix ORCID: 0000-0002-6376-6391, Alpdogan, Serdar, Hescheler, Juergen, Schneider, Toni ORCID: 0000-0003-2816-2696, Albanna, Walid and Akhtar-Schaefer, Isha (2020). Submicromolar copper (II) ions stimulate transretinal signaling in the isolated retina from wild type but not from Ca(v)2.3-deficient mice. BMC Ophthalmol., 20 (1). LONDON: BMC. ISSN 1471-2415

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Abstract

BackgroundSo far, only indirect evidence exists for the pharmacoresistant R-type voltage-gated Ca2+ channel (VGCC) to be involved in transretinal signaling by triggering GABA-release onto ON-bipolar neurons. This release of inhibitory neurotransmitters was deduced from the sensitivity of the b-wave to stimulation by Ni2+, Zn2+ and Cu2+. To further confirm the interpretation of these findings, we compared the effects of Cu2+ application and chelation (using kainic acid, KA) on the neural retina from wildtype and Ca(v)2.3-deficient mice. Furthermore, the immediately effect of KA on the ERG b-wave modulation was assessed.MethodsTransretinal signaling was recorded as an ERG from the superfused murine retina isolated from wildtype and Ca(v)2.3-deficient mice.ResultsIn mice, the stimulating effect of 100nM CuCl2 is absent in the retinae from Ca(v)2.3-deficient mice, but prominent in Ca(v)2.3-competent mice. Application of up to 3mM tricine does not affect the murine b-wave in both genotypes, most likely because of chelating amino acids present in the murine nutrient solution. Application of 27 mu M KA significantly increased the b-wave amplitude in wild type and Ca(v)2.3 (-|-) mice. This effect can most likely be explained by the stimulation of endogenous KA-receptors described in horizontal, OFF-bipolar, amacrine or ganglion cells, which could not be fully blocked in the present study.ConclusionCu(2+)-dependent modulation of transretinal signaling only occurs in the murine retina from Ca(v)2.3 competent mice, supporting the ideas derived from previous work in the bovine retina that R-type Ca2+ channels are involved in shaping transretinal responses during light perception.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Lueke, Jan Niklas UNSPECIFIED UNSPECIFIED UNSPECIFIED Neumaier, Felix UNSPECIFIED orcid.org/0000-0002-6376-6391 UNSPECIFIED Alpdogan, Serdar UNSPECIFIED UNSPECIFIED UNSPECIFIED Hescheler, Juergen UNSPECIFIED UNSPECIFIED UNSPECIFIED Schneider, Toni UNSPECIFIED orcid.org/0000-0003-2816-2696 UNSPECIFIED Albanna, Walid UNSPECIFIED UNSPECIFIED UNSPECIFIED Akhtar-Schaefer, Isha UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-334298
DOI:	10.1186/s12886-020-01451-8
Journal or Publication Title:	BMC Ophthalmol.
Volume:	20
Number:	1
Date:	2020
Publisher:	BMC
Place of Publication:	LONDON
ISSN:	1471-2415
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language B-WAVE AMPLITUDE; CALCIUM-CHANNELS; BOVINE RETINA; BINDING-SITE; ZINC; RECEPTORS; RELEASE; LACKING; EXCITOTOXICITY; DETERMINANTS Multiple languages Ophthalmology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/33429