Brito, Danielle S., Agrimi, Gennaro ORCID: 0000-0002-5219-4412, Charton, Lennart, Brilhaus, Dominik ORCID: 0000-0001-9021-3197, Bitetto, Maria Gabriella, Lana-Costa, Jaciara, Messina, Eugenia, Nascimento, Carolina P., Feitosa-Araujo, Elias, Pires, Marcel Viana, Perez-Diaz, Jorge Luis, Obata, Toshihiro ORCID: 0000-0001-8931-7722, Porcelli, Vito, Palmieri, Luigi, Araujo, Wagner L. ORCID: 0000-0002-4796-2616, Weber, Andreas P. M., Linka, Nicole, Fernie, Alisdair R., Palmieri, Ferdinando and Nunes-Nesi, Adriano (2020). Biochemical and functional characterization of a mitochondrial citrate carrier in Arabidopsis thaliana. Biochem. J., 477 (9). S. 1759 - 1778. LONDON: PORTLAND PRESS LTD. ISSN 1470-8728

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Abstract

A homolog of the mitochondrial succinate/fumarate carrier from yeast (Sfc1p) has been found in the Arabidopsis genome, named AtSFC1. The AtSFC1 gene was expressed in Escherichia coli, and the gene product was purified and reconstituted in liposomes. Its transport properties and kinetic parameters demonstrated that AtSFC1 transports citrate, isocitrate and aconitate and, to a lesser extent, succinate and fumarate. This carrier catalyzes a fast counter-exchange transport as well as a low uniport of substrates, exhibits a higher transport affinity for tricarboxylates than dicarboxylates, and is inhibited by pyridoxal 5'-phosphate and other inhibitors of mitochondrial carriers to various degrees. Gene expression analysis indicated that the AtSFC1 transcript is mainly present in heterotrophic tissues, and fusion with a green-fluorescent protein localized AtSFC1 to the mitochondria. Furthermore, 35S-AtSFC1 antisense lines were generated and characterized at metabolic and physiological levels in different organs and at various developmental stages. Lower expression of AtSFC1 reduced seed germination and impaired radicle growth, a phenotype that was related to reduced respiration rate. These findings demonstrate that AtSFC1 might be involved in storage oil mobilization at the early stages of seedling growth and in nitrogen assimilation in root tissue by catalyzing citrate/isocitrate or citrate/succinate exchanges.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Brito, Danielle S.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Agrimi, GennaroUNSPECIFIEDorcid.org/0000-0002-5219-4412UNSPECIFIED
Charton, LennartUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Brilhaus, DominikUNSPECIFIEDorcid.org/0000-0001-9021-3197UNSPECIFIED
Bitetto, Maria GabriellaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Lana-Costa, JaciaraUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Messina, EugeniaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Nascimento, Carolina P.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Feitosa-Araujo, EliasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Pires, Marcel VianaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Perez-Diaz, Jorge LuisUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Obata, ToshihiroUNSPECIFIEDorcid.org/0000-0001-8931-7722UNSPECIFIED
Porcelli, VitoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Palmieri, LuigiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Araujo, Wagner L.UNSPECIFIEDorcid.org/0000-0002-4796-2616UNSPECIFIED
Weber, Andreas P. M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Linka, NicoleUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Fernie, Alisdair R.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Palmieri, FerdinandoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Nunes-Nesi, AdrianoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-335172
DOI: 10.1042/BCJ20190785
Journal or Publication Title: Biochem. J.
Volume: 477
Number: 9
Page Range: S. 1759 - 1778
Date: 2020
Publisher: PORTLAND PRESS LTD
Place of Publication: LONDON
ISSN: 1470-8728
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
RECONSTITUTED CARNITINE CARRIER; BACTERIAL EXPRESSION; TRICARBOXYLATE CARRIER; SACCHAROMYCES-CEREVISIAE; GLUTAMATE TRANSPORTER; DICARBOXYLATE CARRIER; ORGAN DISTRIBUTION; PHOSPHATE CARRIER; AMINO-ACIDS; COENZYME-AMultiple languages
Biochemistry & Molecular BiologyMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/33517

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