Raspa, Marcello, Paoletti, Renata, Mahabir, Esther and Scavizzi, Ferdinando (2020). D-aspartate treatment in vitro improves mouse sperm fertility in young B6N mice. Theriogenology, 148. S. 60 - 68. NEW YORK: ELSEVIER SCIENCE INC. ISSN 1879-3231

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Abstract

We previously reported that the administration of D-aspartate (D-Asp) in drinking water over a 2-4-week period to 7-week-old mice resulted in higher sperm quality and increased in vitro fertilisation (IVF) rates associated with a systemic increase of luteinizing hormone and testosterone levels in the serum. The goal of this study was to investigate the effects of in vitro treatment with D-Asp on the IVF rate, embryo transfer, and sperm parameters of cryopreserved-thawed C57BL/6NTacCnrm (B6N) spermatozoa derived from young and adult mice. In this study, cryopreserved-thawed B6N spermatozoa from males aging 9, 11, 13, and 16 weeks were treated for 1 h with 4 mM D-Asp during capacitation. Thereafter, the in vitro fertilisation ability and the embryo transfer efficiency were analysed. Also, the kinetic activity of the treated spermatozoa and the acrosome reaction were measured after 1 h, 2 h, and 5 h of incubation. The capacitation rate of spermatozoa was determined after 1 h of pre-incubation. Spermatozoa from 9- and 11-week-old mice, which were treated with D-Asp, led to significantly increased IVF rates. However, spermatozoa derived from 13- and 16-week-old mice did not lead to a significant improvement in the fertilisation rate. At all ages examined, no differences were observed in the birth rate and sperm kinetic parameters. After 1 h incubation under the same conditions as the IVF was performed, the capacitation rate and the acrosome reaction were significantly higher with the D-Asp-treated spermatozoa from 9-week-old (67.5% vs. 41% and 14.5% vs. 10.5%, respectively) and 11-week-old mice (78.5% vs. 41.1% and 21.0% vs. 3.8%, respectively), corresponding to the improved IVF results. Therefore, the present results demonstrate, for the first time, a direct role of D-Asp in the capacitation process and acrosome reaction. (C) 2020 Elsevier Inc. All rights reserved.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Raspa, MarcelloUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Paoletti, RenataUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Mahabir, EstherUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Scavizzi, FerdinandoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-335936
DOI: 10.1016/j.theriogenology.2020.02.031
Journal or Publication Title: Theriogenology
Volume: 148
Page Range: S. 60 - 68
Date: 2020
Publisher: ELSEVIER SCIENCE INC
Place of Publication: NEW YORK
ISSN: 1879-3231
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
PROTECTS BULL SPERM; ACROSOME REACTION; CAPACITATION; SPERMATOZOA; COENZYME-Q10; ACID; ZINC; IDENTIFICATION; CHOLESTEROL; STIMULATEMultiple languages
Reproductive Biology; Veterinary SciencesMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/33593

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