Universität zu Köln

Dabrowski, Rahel, Ripa, Roberto, Latza, Christian, Annibal, Andrea ORCID: 0000-0003-4311-5417 and Antebi, Adam (2020). Optimization of mass spectrometry settings for steroidomic analysis in young and old killifish. Anal. Bioanal. Chem., 412 (17). S. 4089 - 4100. HEIDELBERG: SPRINGER HEIDELBERG. ISSN 1618-2650

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Abstract

Steroids are essential structural components of cell membranes that organize lipid rafts and modulate membrane fluidity. They can also act as signalling molecules that work through nuclear and G protein-coupled receptors to impact health and disease. Notably, changes in steroid levels have been implicated in metabolic, cardiovascular and neurodegenerative diseases, but how alterations in the steroid pool affect ageing is less well understood. One of the major challenges in steroidomic analysis is the ability to simultaneously detect and distinguish various steroids due to low in vivo concentrations and naturally occurring stereoisomers. Here, we established such a method to study the mass spectrometry behaviour of nine sterols/steroids and related molecules (cholesterol precursors: squalene, lanosterol; sterol metabolites; 7 Dehydrocholesterol, 24, 25 and 27 Hydroxycholesterol; and steroids: progesterone, testosterone, and corticosterone) during ageing in the African turquoise killifish, a new model for studying vertebrate longevity. We find that levels of all tested steroids change significantly with age in multiple tissues, suggesting that specific steroids could be used as biomarkers of ageing. These findings pave the way for use of Nothobranchius furzeri as a novel model organism to unravel the role of sterols/steroids in ageing and age-related diseases.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Dabrowski, Rahel UNSPECIFIED UNSPECIFIED UNSPECIFIED Ripa, Roberto UNSPECIFIED UNSPECIFIED UNSPECIFIED Latza, Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED Annibal, Andrea UNSPECIFIED orcid.org/0000-0003-4311-5417 UNSPECIFIED Antebi, Adam UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-336559
DOI:	10.1007/s00216-020-02640-6
Journal or Publication Title:	Anal. Bioanal. Chem.
Volume:	412
Number:	17
Page Range:	S. 4089 - 4100
Date:	2020
Publisher:	SPRINGER HEIDELBERG
Place of Publication:	HEIDELBERG
ISSN:	1618-2650
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SOLID-PHASE EXTRACTION; SIMULTANEOUS QUANTIFICATION; CHOLESTEROL-METABOLISM; FREE CORTISOL; OXYSTEROLS; HORMONES; BRAIN; TESTOSTERONE; ZEBRAFISH; SERUM Multiple languages Biochemical Research Methods; Chemistry, Analytical Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/33655