Universität zu Köln

Lundt, Andreas, Soos, Julien, Seidel, Robin, Henseler, Christina, Mueller, Ralf, Ginde, Varun Raj, Arshaad, Muhammad Imran, Ehninger, Dan, Hescheler, Juergen, Sachinidis, Agapios, Broich, Karl, Wormuth, Carola, Papazoglou, Anna and Weiergraeber, Marco (2020). Functional implications of Ca(v)2.3 R-type voltage-gated calcium channels in the murine auditory system - novel vistas from brainstem-evoked response audiometry. Eur. J. Neurosci., 51 (7). S. 1583 - 1605. HOBOKEN: WILEY. ISSN 1460-9568

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Abstract

Voltage-gated Ca2+ channels (VGCCs) are considered to play a key role in auditory perception and information processing within the murine inner ear and brainstem. In the past, Ca(v)1.3 L-type VGCCs gathered most attention as their ablation causes congenital deafness. However, isolated patch-clamp investigation and localization studies repetitively suggested that Ca(v)2.3 R-type VGCCs are also expressed in the cochlea and further components of the ascending auditory tract, pointing to a potential functional role of Ca(v)2.3 in hearing physiology. Thus, we performed auditory profiling of Ca(v)2.3(+/+) controls, heterozygous Ca(v)2.3(+/-) mice and Ca(v)2.3 null mutants (Ca(v)2.3(-/-)) using brainstem-evoked response audiometry. Interestingly, click-evoked auditory brainstem responses (ABRs) revealed increased hearing thresholds in Ca(v)2.3(+/-) mice from both genders, whereas no alterations were observed in Ca(v)2.3(-/-) mice. Similar observations were made for tone burst-related ABRs in both genders. However, Ca(v)2.3 ablation seemed to prevent mutant mice from total hearing loss particularly in the higher frequency range (36-42 kHz). Amplitude growth function analysis revealed, i.a., significant reduction in ABR wave W-I and W-III amplitude in mutant animals. In addition, alterations in W-I-W-IV interwave interval were observed in female Ca(v)2.3(+/-) mice whereas absolute latencies remained unchanged. In summary, our results demonstrate that Ca(v)2.3 VGCCs are mandatory for physiological auditory information processing in the ascending auditory tract.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Lundt, Andreas UNSPECIFIED UNSPECIFIED UNSPECIFIED Soos, Julien UNSPECIFIED UNSPECIFIED UNSPECIFIED Seidel, Robin UNSPECIFIED UNSPECIFIED UNSPECIFIED Henseler, Christina UNSPECIFIED UNSPECIFIED UNSPECIFIED Mueller, Ralf UNSPECIFIED UNSPECIFIED UNSPECIFIED Ginde, Varun Raj UNSPECIFIED UNSPECIFIED UNSPECIFIED Arshaad, Muhammad Imran UNSPECIFIED UNSPECIFIED UNSPECIFIED Ehninger, Dan UNSPECIFIED UNSPECIFIED UNSPECIFIED Hescheler, Juergen UNSPECIFIED UNSPECIFIED UNSPECIFIED Sachinidis, Agapios UNSPECIFIED UNSPECIFIED UNSPECIFIED Broich, Karl UNSPECIFIED UNSPECIFIED UNSPECIFIED Wormuth, Carola UNSPECIFIED UNSPECIFIED UNSPECIFIED Papazoglou, Anna UNSPECIFIED UNSPECIFIED UNSPECIFIED Weiergraeber, Marco UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-339107
DOI:	10.1111/ejn.14591
Journal or Publication Title:	Eur. J. Neurosci.
Volume:	51
Number:	7
Page Range:	S. 1583 - 1605
Date:	2020
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1460-9568
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CA(V)1.3 CA2+ CHANNELS; INNER HAIR-CELLS; ALPHA-1E SUBUNIT; MICE LACKING; BIOPHYSICAL PROPERTIES; PRESYNAPTIC ACTIVITY; TRANSMITTER RELEASE; HEARING-LOSS; CURRENTS; EXOCYTOSIS Multiple languages Neurosciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/33910