Universität zu Köln

Niedermaier, Stefan, Schneider, Trang, Bahl, Marc-Oliver, Matsubara, Shizue ORCID: 0000-0002-1440-6496 and Huesgen, Pitter F. (2020). Photoprotective Acclimation of the Arabidopsis thaliana Leaf Proteome to Fluctuating Light. Front. Genet., 11. LAUSANNE: FRONTIERS MEDIA SA. ISSN 1664-8021

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Abstract

Plants are subjected to strong fluctuations in light intensity in their natural growth environment, caused both by unpredictable changes due to weather conditions and movement of clouds and upper canopy leaves and predictable changes during day-night cycle. The mechanisms of long-term acclimation to fluctuating light (FL) are still not well understood. Here, we used quantitative mass spectrometry to investigate long-term acclimation of low light-grown Arabidopsis thaliana to a FL condition that induces mild photooxidative stress. On the third day of exposure to FL, young and mature leaves were harvested in the morning and at the end of day for proteome analysis using a stable isotope labeling approach. We identified 2,313 proteins, out of which 559 proteins exhibited significant changes in abundance in at least one of the four experimental groups (morning-young, morning-mature, end-of-day-young, end-of-day-mature). A core set of 49 proteins showed significant responses to FL in three or four experimental groups, which included enhanced accumulation of proteins involved in photoprotection, cyclic electron flow around photosystem I, photorespiration, and glycolysis, while specific glutathione transferases and proteins involved in translation and chlorophyll biosynthesis were reduced in abundance. In addition, we observed pathway- and protein-specific changes predominantly at the end of day, whereas few changes were observed exclusively in the morning. Comparison of the proteome data with the matching transcript data revealed gene- and protein-specific responses, with several chloroplast-localized proteins decreasing in abundance despite increased gene expression under FL. Together, our data shows moderate but widespread alterations of protein abundance during acclimation to FL and suggests an important role of post-transcriptional regulation of protein abundance.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Niedermaier, Stefan UNSPECIFIED UNSPECIFIED UNSPECIFIED Schneider, Trang UNSPECIFIED UNSPECIFIED UNSPECIFIED Bahl, Marc-Oliver UNSPECIFIED UNSPECIFIED UNSPECIFIED Matsubara, Shizue UNSPECIFIED orcid.org/0000-0002-1440-6496 UNSPECIFIED Huesgen, Pitter F. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-341402
DOI:	10.3389/fgene.2020.00154
Journal or Publication Title:	Front. Genet.
Volume:	11
Date:	2020
Publisher:	FRONTIERS MEDIA SA
Place of Publication:	LAUSANNE
ISSN:	1664-8021
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CYCLIC ELECTRON FLOW; PHOTOSYSTEM-II; D1 PROTEIN; GROWTH; GENE; DEGRADATION; ENRICHMENT; PLANTS; PHOTOINHIBITION; PHOTOSYNTHESIS Multiple languages Genetics & Heredity Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/34140