Universität zu Köln

Byrneee, Adam J., Poweile, Joseph E., OSeiyan, Brendan J., Oggeri, Patricia P., Hoffland, Ashley ORCID: 0000-0003-2051-9363, Cook, James, Bonner, Katie L., Hewitt, Richard J., Wolf, Simone ORCID: 0000-0003-2073-8312, Ghai, Poonam, Walker, Simone A., Lukowski, Samuel W., Molyneaux, Philip L., Saglani, Sejal, Chambers, Daniel C., Maher, Toby M. and Lloyd, Clare M. (2020). Dynamics of human monocytes and airway macrophages during healthy aging and after transplant. J. Exp. Med., 217 (3). NEW YORK: ROCKEFELLER UNIV PRESS. ISSN 1540-9538

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Abstract

The ontogeny of airway macrophages (AMs) in human lung and their contribution to disease are poorly mapped out. In mice, aging is associated with an increasing proportion of peripherally, as opposed to perinatally derived AMs. We sought to understand AM ontogeny in human lung during healthy aging and after transplant. We characterized monocyte/macrophage populations from the peripheral blood and airways of healthy volunteers across infancy/childhood (2-12 yr), maturity (20-50 yr), and older adulthood (>50 yr). Single-cell RNA sequencing (scRNA-seq) was performed on airway inflammatory cells isolated from sex-mismatched lung transplant recipients. During healthy aging, the proportions of blood and bronchoalveolar lavage (BAL) classical monocytes peak in adulthood and decline in older adults. scRNA-seq of BAL cells from lung transplant recipients indicates that after transplant, the majority of AMs are recipient derived. These data show that during aging, the peripheral monocyte phenotype is consistent with that found in the airways and, furthermore, that the majority of human AMs after transplant are derived from circulating monocytes.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Byrneee, Adam J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Poweile, Joseph E. UNSPECIFIED UNSPECIFIED UNSPECIFIED OSeiyan, Brendan J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Oggeri, Patricia P. UNSPECIFIED UNSPECIFIED UNSPECIFIED Hoffland, Ashley UNSPECIFIED orcid.org/0000-0003-2051-9363 UNSPECIFIED Cook, James UNSPECIFIED UNSPECIFIED UNSPECIFIED Bonner, Katie L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Hewitt, Richard J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Wolf, Simone UNSPECIFIED orcid.org/0000-0003-2073-8312 UNSPECIFIED Ghai, Poonam UNSPECIFIED UNSPECIFIED UNSPECIFIED Walker, Simone A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Lukowski, Samuel W. UNSPECIFIED UNSPECIFIED UNSPECIFIED Molyneaux, Philip L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Saglani, Sejal UNSPECIFIED UNSPECIFIED UNSPECIFIED Chambers, Daniel C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Maher, Toby M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Lloyd, Clare M. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-342496
DOI:	10.1084/jem.20191236
Journal or Publication Title:	J. Exp. Med.
Volume:	217
Number:	3
Date:	2020
Publisher:	ROCKEFELLER UNIV PRESS
Place of Publication:	NEW YORK
ISSN:	1540-9538
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ALVEOLAR MACROPHAGES; PULMONARY MACROPHAGES; BONE-MARROW; LUNG; CELLS; LIFE; FIBROSIS; ONTOGENY; DEVELOP; ORIGIN Multiple languages Immunology; Medicine, Research & Experimental Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/34249