Fernandez-Rebollo, Eduardo, Franzen, Julia, Goetzke, Roman ORCID: 0000-0002-1845-1083, Hollmann, Jonathan, Ostrowska, Alina, Oliverio, Matteo, Sieben, Torsten, Rath, Bjoern, Kornfeld, Jan-Wilhelm ORCID: 0000-0002-6802-4442 and Wagner, Wolfgang ORCID: 0000-0002-1971-3217 (2020). Senescence-Associated Metabolomic Phenotype in Primary and iPSC-Derived Mesenchymal Stromal Cells. Stem Cell Rep., 14 (2). S. 201 - 210. CAMBRIDGE: CELL PRESS. ISSN 2213-6711
Full text not available from this repository.Abstract
Long-term culture of primary cells is characterized by functional and secretory changes, which ultimately result in replicative senescence. It is largely unclear how the metabolome of cells changes during replicative senescence and if such changes are consistent across different cell types. We have directly compared culture expansion of primary mesenchymal stromal cells (MSCs) and induced pluripotent stem cell-derived MSCs (iMSCs) until they reached growth arrest. Both cell types acquired similar changes in morphology, in vitro differentiation potential, senescence-associated beta-galactosidase, and DNA methylation. Furthermore, MSCs and iMSCs revealed overlapping gene expression changes, particularly in functional categories related to metabolic processes. We subsequently compared the metabolomes of MSCs and iMSCs and observed overlapping senescence-associated changes in both cell types, including downregulation of nicotinamide ribonucleotide and upregulation of orotic acid. Taken together, replicative senescence is associated with a highly reproducible senescence-associated metabolomics phenotype, which may be used to monitor the state of cellular aging.
Item Type: | Journal Article | ||||||||||||||||||||||||||||||||||||||||||||
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URN: | urn:nbn:de:hbz:38-345046 | ||||||||||||||||||||||||||||||||||||||||||||
DOI: | 10.1016/j.stemcr.2019.12.012 | ||||||||||||||||||||||||||||||||||||||||||||
Journal or Publication Title: | Stem Cell Rep. | ||||||||||||||||||||||||||||||||||||||||||||
Volume: | 14 | ||||||||||||||||||||||||||||||||||||||||||||
Number: | 2 | ||||||||||||||||||||||||||||||||||||||||||||
Page Range: | S. 201 - 210 | ||||||||||||||||||||||||||||||||||||||||||||
Date: | 2020 | ||||||||||||||||||||||||||||||||||||||||||||
Publisher: | CELL PRESS | ||||||||||||||||||||||||||||||||||||||||||||
Place of Publication: | CAMBRIDGE | ||||||||||||||||||||||||||||||||||||||||||||
ISSN: | 2213-6711 | ||||||||||||||||||||||||||||||||||||||||||||
Language: | English | ||||||||||||||||||||||||||||||||||||||||||||
Faculty: | Unspecified | ||||||||||||||||||||||||||||||||||||||||||||
Divisions: | Unspecified | ||||||||||||||||||||||||||||||||||||||||||||
Subjects: | no entry | ||||||||||||||||||||||||||||||||||||||||||||
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URI: | http://kups.ub.uni-koeln.de/id/eprint/34504 |
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