Fernandez-Rebollo, Eduardo, Franzen, Julia, Goetzke, Roman ORCID: 0000-0002-1845-1083, Hollmann, Jonathan, Ostrowska, Alina, Oliverio, Matteo, Sieben, Torsten, Rath, Bjoern, Kornfeld, Jan-Wilhelm ORCID: 0000-0002-6802-4442 and Wagner, Wolfgang ORCID: 0000-0002-1971-3217 (2020). Senescence-Associated Metabolomic Phenotype in Primary and iPSC-Derived Mesenchymal Stromal Cells. Stem Cell Rep., 14 (2). S. 201 - 210. CAMBRIDGE: CELL PRESS. ISSN 2213-6711

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Abstract

Long-term culture of primary cells is characterized by functional and secretory changes, which ultimately result in replicative senescence. It is largely unclear how the metabolome of cells changes during replicative senescence and if such changes are consistent across different cell types. We have directly compared culture expansion of primary mesenchymal stromal cells (MSCs) and induced pluripotent stem cell-derived MSCs (iMSCs) until they reached growth arrest. Both cell types acquired similar changes in morphology, in vitro differentiation potential, senescence-associated beta-galactosidase, and DNA methylation. Furthermore, MSCs and iMSCs revealed overlapping gene expression changes, particularly in functional categories related to metabolic processes. We subsequently compared the metabolomes of MSCs and iMSCs and observed overlapping senescence-associated changes in both cell types, including downregulation of nicotinamide ribonucleotide and upregulation of orotic acid. Taken together, replicative senescence is associated with a highly reproducible senescence-associated metabolomics phenotype, which may be used to monitor the state of cellular aging.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Fernandez-Rebollo, EduardoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Franzen, JuliaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Goetzke, RomanUNSPECIFIEDorcid.org/0000-0002-1845-1083UNSPECIFIED
Hollmann, JonathanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ostrowska, AlinaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Oliverio, MatteoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Sieben, TorstenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Rath, BjoernUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kornfeld, Jan-WilhelmUNSPECIFIEDorcid.org/0000-0002-6802-4442UNSPECIFIED
Wagner, WolfgangUNSPECIFIEDorcid.org/0000-0002-1971-3217UNSPECIFIED
URN: urn:nbn:de:hbz:38-345046
DOI: 10.1016/j.stemcr.2019.12.012
Journal or Publication Title: Stem Cell Rep.
Volume: 14
Number: 2
Page Range: S. 201 - 210
Date: 2020
Publisher: CELL PRESS
Place of Publication: CAMBRIDGE
ISSN: 2213-6711
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
DNA-METHYLATION; INCREASED GLYCOLYSIS; CELLULAR SENESCENCE; STEM-CELLSMultiple languages
Cell & Tissue Engineering; Cell BiologyMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/34504

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