Universität zu Köln

He, Xuan, Wedekind, Franziska, Kroll, Tina, Oskamp, Angela, Beer, Simone, Drzezga, Alexander, Ermert, Johannes ORCID: 0000-0002-2561-7766, Neumaier, Bernd, Bauer, Andreas and Elmenhorst, David (2020). Image-Derived Input Functions for Quantification of A(1) Adenosine Receptors Availability in Mice Brains Using PET and [F-18]CPFPX. Front. Physiol., 10. LAUSANNE: FRONTIERS MEDIA SA. ISSN 1664-042X

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Abstract

Purpose In vivo imaging for the A(1) adenosine receptors (A(1)ARs) with positron emission tomography (PET) using 8-cyclopentyl-3-(3-[F-18]fluoropropyl)-1-propylxan- thine ([F-18]CPFPX) has become an important tool for studying physiological processes quantitatively in mice. However, the measurement of arterial input functions (AIFs) on mice is a method with restricted applicability because of the small total blood volume and the related difficulties in withdrawing blood. Therefore, the aim of this study was to extract an appropriate [F-18]CPFPX image-derived input function (IDIF) from dynamic PET images of mice. Procedures In this study, five mice were scanned with [F-18]CPFPX for 60 min. Arterial blood samples (n = 7 per animal) were collected from the femoral artery and corrected for metabolites. To generate IDIFs, three different approaches were selected: (A) volume of interest (VOI) placed over the heart (cube, 10 mm); (B) VOI set over abdominal vena cava/aorta region with a cuboid (5 x 5 x 15 mm); and (C) with 1 x 1 x 1 mm voxels on five consecutive slices. A calculated scaling factor (alpha) was used to correct for partial volume effect; the method of obtaining the total metabolite correction of [F-18]CPFPX for IDIFs was developed. Three IDIFs were validated by comparison with AIF. Validation included the following: visual performance; computing area under the curve (AUC) ratios (IDIF/AIF) of whole-blood curves and parent curves; and the mean distribution volume (V-T) ratios (IDIF/AIF) of A(1)ARs calculated by Logan plot and two-tissue compartment model. Results Compared with the AIF, the IDIF with VOI over heart showed the best performance among the three IDIFs after scaling by 1.77 (alpha) in terms of visual analysis, AUC ratios (IDIF/AIF; whole-blood AUC ratio, 1.03 +/- 0.06; parent curve AUC ratio, 1.01 +/- 0.10) and V-T ratios (IDIF/AIF; Logan V-T ratio, 1.00 +/- 0.17; two-tissue compartment model V-T ratio, 1.00 +/- 0.13) evaluation. The A(1)ARs distribution of average parametric images was in good accordance to autoradiography of the mouse brain. Conclusion The proposed study provides evidence that IDIF with VOI over heart can replace AIF effectively for quantification of A(1)ARs using PET and [F-18]CPFPX in mice brains.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code He, Xuan UNSPECIFIED UNSPECIFIED UNSPECIFIED Wedekind, Franziska UNSPECIFIED UNSPECIFIED UNSPECIFIED Kroll, Tina UNSPECIFIED UNSPECIFIED UNSPECIFIED Oskamp, Angela UNSPECIFIED UNSPECIFIED UNSPECIFIED Beer, Simone UNSPECIFIED UNSPECIFIED UNSPECIFIED Drzezga, Alexander UNSPECIFIED UNSPECIFIED UNSPECIFIED Ermert, Johannes UNSPECIFIED orcid.org/0000-0002-2561-7766 UNSPECIFIED Neumaier, Bernd UNSPECIFIED UNSPECIFIED UNSPECIFIED Bauer, Andreas UNSPECIFIED UNSPECIFIED UNSPECIFIED Elmenhorst, David UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-348022
DOI:	10.3389/fphys.2019.01617
Journal or Publication Title:	Front. Physiol.
Volume:	10
Date:	2020
Publisher:	FRONTIERS MEDIA SA
Place of Publication:	LAUSANNE
ISSN:	1664-042X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language POSITRON-EMISSION-TOMOGRAPHY; SMALL-ANIMAL PET; PARTIAL-VOLUME CORRECTION; CEREBRAL-BLOOD-FLOW; TIME-ACTIVITY CURVE; NONINVASIVE QUANTIFICATION; DYNAMIC PET; METABOLISM; MICROPET; LIGAND Multiple languages Physiology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/34802