Universität zu Köln

Koprivova, Anna, Schmalenberger, Achim and Kopriva, Stanislav ORCID: 0000-0002-7416-6551 (2020). Sulfatase Assay to Determine Influence of Plants on Microbial Activity in Soil. Bio-protocol, 10 (2). SUNNYVALE: BIO-PROTOCOL. ISSN 2331-8325

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Abstract

Sulfatase activity is often used as a measure of the activity of soil microorganisms. It is thus a suitable tool to investigate the response of microbes to plants. Here we present a method to determine the influence of various Arabidopsis genotypes on the function of soil microbiota using the sulfatase as a quantitative measure. We grew the plants in soil/sand mix under control conditions and measured the sulfatase activity in soil using a spectrophotometric determination of the product. This protocol can be used to test the contribution of individual genes to control of microbiome assembly through analysis of mutants as well as the influence of environment on plant-microbe interactions.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Koprivova, Anna UNSPECIFIED UNSPECIFIED UNSPECIFIED Schmalenberger, Achim UNSPECIFIED UNSPECIFIED UNSPECIFIED Kopriva, Stanislav UNSPECIFIED orcid.org/0000-0002-7416-6551 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-348509
DOI:	10.21769/BioProtoc.3490
Journal or Publication Title:	Bio-protocol
Volume:	10
Number:	2
Date:	2020
Publisher:	BIO-PROTOCOL
Place of Publication:	SUNNYVALE
ISSN:	2331-8325
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/34850