Universität zu Köln

Tröder, Simon Erwin ORCID: 0000-0003-1156-2976 and Zevnik, Branko ORCID: 0000-0001-7845-9522 (2021). History of genome editing: From meganucleases to CRISPR. Laboratory Animals. ISSN 1758-1117

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Abstract

CRISPR-mediated genome editing has undoubtedly revolutionized genetic engineering of animals. With the ability for virtually unlimited modification of almost any genome it is easy to forget which amazing discoveries paved the way for this groundbreaking technology. Here, we summarize the history of genome editing platforms, starting from enhanced integration of foreign DNA by meganuclease-mediated double strand breaks to CRISPR/Cas9, the leading technology to date, and its re-engineered variants.

Item Type:	Article
Creators:	Creators Email ORCID ORCID Put Code Tröder, Simon Erwin simon.troeder@uk-koeln.de https://orcid.org/0000-0003-1156-2976 UNSPECIFIED Zevnik, Branko branko.zevnik@uk-koeln.de https://orcid.org/0000-0001-7845-9522 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-368221
Identification Number:	10.1177/0023677221994613
Journal or Publication Title:	Laboratory Animals
Date:	23 February 2021
ISSN:	1758-1117
Language:	English
Faculty:	Faculty of Medicine
Divisions:	CECAD - Cluster of Excellence Cellular Stress Responses in Aging-Associated Diseases
Subjects:	Life sciences
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/36822