Universität zu Köln

Freudenberg, Uwe ORCID: 0000-0002-1359-1095, Zieris, Andrea, Chwalek, Karolina ORCID: 0000-0002-8947-5605, Tsurkan, Mikhail V. ORCID: 0000-0003-4000-3890, Maitz, Manfred F., Atallah, Passant, Levental, Kandice R., Eming, Sabine A. and Werner, Carsten ORCID: 0000-0003-0189-3448 (2015). Heparin desulfation modulates VEGF release and angiogenesis in diabetic wounds. J. Control. Release, 220. S. 79 - 89. AMSTERDAM: ELSEVIER. ISSN 1873-4995

Full text not available from this repository.

Abstract

While vascular endothelial growth factor (VEGF) has been shown to be one of the key players in wound healing by promoting angiogenesis current clinical applications of this growth factor to the wound environment are poorly controlled and not sustainable. Hydrogels made of sulfated glycosaminoglycans (GAG) allow for the sustained release of growth factors since GAGs engage in electrostatic complexation of biomolecules. In here, we explore a set of hydrogels formed of selectively desulfated heparin derivatives and star-shaped poly(ethylene glycol) with respect to VEGF binding and release and anticoagulant activity. As a proof of concept, supportive effects on migration and tube formation of human umbilical vein endothelial cells were studied in vitro and the promotion of wound healing was followed in genetically diabetic (db/db) mice. Our data demonstrate that the release of VEGF from the hydrogels is modulated in dependence on the GAG sulfation pattern. Hydrogels with low sulfate content (11% of initial heparin) were found to be superior in efficacy of VEGF administration, low anticoagulant activity and promotion of angiogenesis. (C) 2015 Elsevier B.V. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Freudenberg, Uwe UNSPECIFIED orcid.org/0000-0002-1359-1095 UNSPECIFIED Zieris, Andrea UNSPECIFIED UNSPECIFIED UNSPECIFIED Chwalek, Karolina UNSPECIFIED orcid.org/0000-0002-8947-5605 UNSPECIFIED Tsurkan, Mikhail V. UNSPECIFIED orcid.org/0000-0003-4000-3890 UNSPECIFIED Maitz, Manfred F. UNSPECIFIED UNSPECIFIED UNSPECIFIED Atallah, Passant UNSPECIFIED UNSPECIFIED UNSPECIFIED Levental, Kandice R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Eming, Sabine A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Werner, Carsten UNSPECIFIED orcid.org/0000-0003-0189-3448 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-383497
DOI:	10.1016/j.jconrel.2015.10.028
Journal or Publication Title:	J. Control. Release
Volume:	220
Page Range:	S. 79 - 89
Date:	2015
Publisher:	ELSEVIER
Place of Publication:	AMSTERDAM
ISSN:	1873-4995
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ENDOTHELIAL GROWTH-FACTOR; DELIVERY; HYDROGEL; ADHESION; BINDING; WATER Multiple languages Chemistry, Multidisciplinary; Pharmacology & Pharmacy Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/38349