Universität zu Köln

Klecker, Till ORCID: 0000-0003-0149-042X, Wemmer, Megan, Haag, Mathias, Weig, Alfons, Boeckler, Stefan, Langer, Thomas ORCID: 0000-0003-1250-1462, Nunnari, Jodi and Westermann, Benedikt ORCID: 0000-0002-2991-1604 (2015). Interaction of MDM33 with mitochondrial inner membrane homeostasis pathways in yeast. Sci Rep, 5. LONDON: NATURE PUBLISHING GROUP. ISSN 2045-2322

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Abstract

Membrane homeostasis affects mitochondrial dynamics, morphology, and function. Here we report genetic and proteomic data that reveal multiple interactions of Mdm33, a protein essential for normal mitochondrial structure, with components of phospholipid metabolism and mitochondrial inner membrane homeostasis. We screened for suppressors of MDM33 overexpression-induced growth arrest and isolated binding partners by immunoprecipitation of cross-linked cell extracts. These approaches revealed genetic and proteomic interactions of Mdm33 with prohibitins, Phb1 and Phb2, which are key components of mitochondrial inner membrane homeostasis. Lipid profiling by mass spectrometry of mitochondria isolated from Mdm33-overexpressing cells revealed that high levels of Mdm33 affect the levels of phosphatidylethanolamine and cardiolipin, the two key inner membrane phospholipids. Furthermore, we show that cells lacking Mdm33 show strongly decreased mitochondrial fission activity indicating that Mdm33 is critical for mitochondrial membrane dynamics. Our data suggest that MDM33 functionally interacts with components important for inner membrane homeostasis and thereby supports mitochondrial division.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Klecker, Till UNSPECIFIED orcid.org/0000-0003-0149-042X UNSPECIFIED Wemmer, Megan UNSPECIFIED UNSPECIFIED UNSPECIFIED Haag, Mathias UNSPECIFIED UNSPECIFIED UNSPECIFIED Weig, Alfons UNSPECIFIED UNSPECIFIED UNSPECIFIED Boeckler, Stefan UNSPECIFIED UNSPECIFIED UNSPECIFIED Langer, Thomas UNSPECIFIED orcid.org/0000-0003-1250-1462 UNSPECIFIED Nunnari, Jodi UNSPECIFIED UNSPECIFIED UNSPECIFIED Westermann, Benedikt UNSPECIFIED orcid.org/0000-0002-2991-1604 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-383668
DOI:	10.1038/srep18344
Journal or Publication Title:	Sci Rep
Volume:	5
Date:	2015
Publisher:	NATURE PUBLISHING GROUP
Place of Publication:	LONDON
ISSN:	2045-2322
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SYNTHETIC LETHAL INTERACTION; INTERMEMBRANE-SPACE-PROTEIN; DYNAMIN-RELATED GTPASE; CYTOCHROME-C-OXIDASE; SACCHAROMYCES-CEREVISIAE; PHOSPHATIDYLSERINE DECARBOXYLASE; ER-MITOCHONDRIA; BARTH SYNDROME; CARDIOLIPIN; FUSION Multiple languages Multidisciplinary Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/38366