Universität zu Köln

Sirker, M., Liang, W., Zhang, L., Fimmers, R., Rothschild, A., Gomes, I. and Schneider, M. (2015). Impact of using validated or standard reference genes for miRNA qPCR data normalization in cell type identification. Forensic Sci. Int. Genet. Suppl. Ser., 5. S. E199 - 3. CLARE: ELSEVIER IRELAND LTD. ISSN 1875-175X

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Abstract

For the analysis of cell type-specific miRNA expression patterns qPCR is currently the method of choice owing to its high accuracy. However, to obtain reliable results, a proper normalization strategy is an absolute prerequisite, which is often underestimated. To demonstrate the importance of using a set of suitable reference genes, we tested two normalization strategies by comparing gene expressions of tissue-specific miRNA targets normalized against: (1) previously validated endogenous controls (miR92 and miR374) and (2) a commonly used miRNA reference (U6B). (C) 2015 Elsevier Ireland Ltd. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Sirker, M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Liang, W. UNSPECIFIED UNSPECIFIED UNSPECIFIED Zhang, L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Fimmers, R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Rothschild, A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Gomes, I. UNSPECIFIED UNSPECIFIED UNSPECIFIED Schneider, M. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-384494
DOI:	10.1016/j.fsigss.2015.09.080
Journal or Publication Title:	Forensic Sci. Int. Genet. Suppl. Ser.
Volume:	5
Page Range:	S. E199 - 3
Date:	2015
Publisher:	ELSEVIER IRELAND LTD
Place of Publication:	CLARE
ISSN:	1875-175X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language QUANTITATIVE PCR DATA Multiple languages Genetics & Heredity Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/38449