Universität zu Köln

Eisenhardt, Nathalie, Chaugule, Viduth K. ORCID: 0000-0001-7814-5865, Koidl, Stefanie, Droescher, Mathias, Dogan, Esen, Rettich, Jan, Sutinen, Paeivi, Imanishi, Susumu Y., Hofmann, Kay ORCID: 0000-0002-2289-9083, Palvimo, Jorma J. and Pichler, Andrea (2015). A new vertebrate SUMO enzyme family reveals insights into SUMO-chain assembly. Nat. Struct. Mol. Biol., 22 (12). S. 959 - 968. NEW YORK: NATURE PUBLISHING GROUP. ISSN 1545-9985

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Abstract

SUMO chains act as stress-induced degradation tags or repair factor-recruiting signals at DNA lesions. Although El activating, E2 conjugating and E3 ligating enzymes efficiently assemble SUMO chains, specific chain-elongation mechanisms are unknown. E4 elongases are specialized E3 ligases that extend a chain but are inefficient in the initial conjugation of the modifier. We identified ZNF451, a representative member of a new class of SUMO2 and SUM03 (SUMO2/3)-specific enzymes that execute catalysis via a tandem SUMO-interaction motif (SIM) region. One SIM positions the donor SUMO while a second SIM binds SUMO on the back side of the E2 enzyme. This tandem-SIM region is sufficient to extend a back side-anchored SUMO chain (E4 elongase activity), whereas efficient chain initiation also requires a zinc-finger region to recruit the initial acceptor SUMO (E3 ligase activity). Finally, we describe four human proteins sharing E4 elongase activities and their function in stress-induced SUMO2/3 conjugation.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Eisenhardt, Nathalie UNSPECIFIED UNSPECIFIED UNSPECIFIED Chaugule, Viduth K. UNSPECIFIED orcid.org/0000-0001-7814-5865 UNSPECIFIED Koidl, Stefanie UNSPECIFIED UNSPECIFIED UNSPECIFIED Droescher, Mathias UNSPECIFIED UNSPECIFIED UNSPECIFIED Dogan, Esen UNSPECIFIED UNSPECIFIED UNSPECIFIED Rettich, Jan UNSPECIFIED UNSPECIFIED UNSPECIFIED Sutinen, Paeivi UNSPECIFIED UNSPECIFIED UNSPECIFIED Imanishi, Susumu Y. UNSPECIFIED UNSPECIFIED UNSPECIFIED Hofmann, Kay UNSPECIFIED orcid.org/0000-0002-2289-9083 UNSPECIFIED Palvimo, Jorma J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Pichler, Andrea UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-385267
DOI:	10.1038/nsmb.3114
Journal or Publication Title:	Nat. Struct. Mol. Biol.
Volume:	22
Number:	12
Page Range:	S. 959 - 968
Date:	2015
Publisher:	NATURE PUBLISHING GROUP
Place of Publication:	NEW YORK
ISSN:	1545-9985
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Institute for Genetics
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language TARGETED UBIQUITIN LIGASE; E3 LIGASE; SLX4 COMPLEX; DNA-DAMAGE; UBC9; SUMOYLATION; RNF4; MECHANISM; IDENTIFICATION; RANBP2 Multiple languages Biochemistry & Molecular Biology; Biophysics; Cell Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/38526