Universität zu Köln

Schacherl, Magdalena ORCID: 0000-0002-5478-2509, Montada, Angelika A. M., Brunstein, Elena and Baumann, Ulrich (2015). The first crystal structure of the peptidase domain of the U32 peptidase family. Acta Crystallogr. Sect. D-Struct. Biol., 71. S. 2505 - 2513. CHESTER: INT UNION CRYSTALLOGRAPHY. ISSN 2059-7983

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Abstract

The U32 family is a collection of over 2500 annotated peptidases in the MEROPS database with unknown catalytic mechanism. They mainly occur in bacteria and archaea, but a few representatives have also been identified in eukarya. Many of the U32 members have been linked to pathogenicity, such as proteins from Helicobacter and Salmonella. The first crystal structure analysis of a U32 catalytic domain from Methanopyrus kandleri (gene mk0906) reveals a modified (beta alpha) 8 TIM-barrel fold with some unique features. The connecting segment between strands beta 7 and beta 8 is extended and helix alpha 7 is located on top of the C-terminal end of the barrel body. The protein exhibits a dimeric quaternary structure in which a zinc ion is symmetrically bound by histidine and cysteine side chains from both monomers. These residues reside in conserved sequence motifs. No typical proteolytic motifs are discernible in the three-dimensional structure, and biochemical assays failed to demonstrate proteolytic activity. A tunnel in which an acetate ion is bound is located in the C-terminal part of the beta-barrel. Two hydrophobic grooves lead to a tunnel at the C-terminal end of the barrel in which an acetate ion is bound. One of the grooves binds to a Strep-Tag II of another dimer in the crystal lattice. Thus, these grooves may be binding sites for hydrophobic peptides or other ligands.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Schacherl, Magdalena UNSPECIFIED orcid.org/0000-0002-5478-2509 UNSPECIFIED Montada, Angelika A. M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Brunstein, Elena UNSPECIFIED UNSPECIFIED UNSPECIFIED Baumann, Ulrich UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-385697
DOI:	10.1107/S1399004715019549
Journal or Publication Title:	Acta Crystallogr. Sect. D-Struct. Biol.
Volume:	71
Page Range:	S. 2505 - 2513
Date:	2015
Publisher:	INT UNION CRYSTALLOGRAPHY
Place of Publication:	CHESTER
ISSN:	2059-7983
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Chemistry > Institute of Biochemistry
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SEQUENCE; COLLAGENASE; EXPRESSION; GENE; IDENTIFICATION; SALMONELLA; VIRULENCE Multiple languages Biochemical Research Methods; Biochemistry & Molecular Biology; Biophysics; Crystallography Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/38569