Universität zu Köln

Knabl, Julia, Hiden, Ursula, Huettenbrenner, Rebecca, Riedel, Christina, Hutter, Stefan, Kirn, Verena, Guenthner-Biller, Margit, Desoye, Gernot ORCID: 0000-0002-5715-3230, Kainer, Franz and Jeschke, Udo (2015). GDM Alters Expression of Placental Estrogen Receptor in a Cell Type and Gender-Specific Manner. Reprod. Sci., 22 (12). S. 1488 - 1496. HEIDELBERG: SPRINGER HEIDELBERG. ISSN 1933-7205

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Abstract

Objective: The nuclear receptor estrogen receptor (ER) is one of the key players in energy balance, insulin resistance, and trophoblast differentiation. We tested the hypothesis that gestational diabetes mellitus (GDM) alters expression of placental ER in a cell type-specific manner and that this regulation may involve epigenetic changes. Study Design: Expression of ER was analyzed by immunohistochemistry using the semiquantitative immunoreactive score in 80 placentas (40 GDM/40 controls). Quantitative real-time polymerase chain reaction (PCR) measured ER messenger RNA (mRNA) in decidual tissue. Methylation-specific PCR was performed to analyze cytosine-phosphatidyl-guanine-island methylation of the ER promoter. Results: Expression of ER protein is upregulated (P = .011) in GDM in extravillous trophoblasts but not in syncytiotrophoblast. Gestational diabetes mellitus downregulated ER in decidual vessels only in pregnancies with male but not female fetuses. Furthermore, mRNA of the ER encoding gene estrogen receptor gene 1 (ESR1) was increased (+1.77 fold) in GDM decidua when compared to controls (P = .024). In parallel, the promoter of ESR1 was methylated only in decidua of healthy control individuals but not in GDM. Conclusion: Gestational diabetes mellitus affects expression of placental ER in a cell type-dependent way, on epigenetic level. These data link GDM with epigenetic deregulations of ER expression and open new insights into the intrauterine programming hypothesis of GDM.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Knabl, Julia UNSPECIFIED UNSPECIFIED UNSPECIFIED Hiden, Ursula UNSPECIFIED UNSPECIFIED UNSPECIFIED Huettenbrenner, Rebecca UNSPECIFIED UNSPECIFIED UNSPECIFIED Riedel, Christina UNSPECIFIED UNSPECIFIED UNSPECIFIED Hutter, Stefan UNSPECIFIED UNSPECIFIED UNSPECIFIED Kirn, Verena UNSPECIFIED UNSPECIFIED UNSPECIFIED Guenthner-Biller, Margit UNSPECIFIED UNSPECIFIED UNSPECIFIED Desoye, Gernot UNSPECIFIED orcid.org/0000-0002-5715-3230 UNSPECIFIED Kainer, Franz UNSPECIFIED UNSPECIFIED UNSPECIFIED Jeschke, Udo UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-386401
DOI:	10.1177/1933719115585147
Journal or Publication Title:	Reprod. Sci.
Volume:	22
Number:	12
Page Range:	S. 1488 - 1496
Date:	2015
Publisher:	SPRINGER HEIDELBERG
Place of Publication:	HEIDELBERG
ISSN:	1933-7205
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language GESTATIONAL DIABETES-MELLITUS; VITAMIN-D-RECEPTOR; DNA METHYLATION; GENE-EXPRESSION; ER-ALPHA; TROPHOBLAST; PROMOTER; OBESITY; MECHANISMS; EXPOSURE Multiple languages Obstetrics & Gynecology; Reproductive Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/38640