Universität zu Köln

Kloess, Stephan, Chambron, Nicole, Gardlowski, Tanja, Weil, Sandra, Koch, Joachim, Esser, Ruth, von Strandmann, Elke Pogge, Morgan, Michael A., Arseniev, Lubomir, Seitz, Oliver and Koehl, Ulrike (2015). Cetuximab reconstitutes pro-inflammatory cytokine secretions and tumor-infiltrating capabilities of sMICA-inhibited NK cells in HNSCC tumor spheroids. Front. Immunol., 6. LAUSANNE: FRONTIERS MEDIA SA. ISSN 1664-3224

Full text not available from this repository.

Abstract

Immunosuppressive factors, such as soluble major histocompatibility complex class I chain-related peptide A (sMICA) and transforming growth factor beta 1 (TGF-beta(1)), are involved in tumor immune escape mechanisms (TIEMs) exhibited by head and neck squamous cell carcinomas (HNSCCs) and may represent opportunities for therapeutic intervention. In order to overcome TIEMs, we investigated the antibody-dependent cellular cytotoxicity (ADCC), cytokine release and retargeted tumor infiltration of sMICA-inhibited patient NK cells expressing Fc gamma receptor Illa (Fc gamma RIlla, CD16a) in the presence of cetuximab, an anti-epidermal growth factor receptor (HER1) monoclonal antibody (mAb). Compared to healthy controls, relapsed HNSCC patients (n = 5), not currently in treatment revealed decreased levels of circulating regulatory NK cell subsets in relation to increased cytotoxic NK cell subpopulations. Elevated sMICA and TGF-beta(1) plasma levels correlated with diminished TNF alpha and IFN-gamma release and decreased NKG2D (natural killer group 2 member D)-dependent killing of HNSCC cells by NK cells. Incubation of IL-2-activated patient NK cells with patient plasma containing elevated sMICA or sMICA analogs (shed MICA and recombinant MICA) significantly impaired NKG2D-mediated killing by down-regulation of NKG2D surface expression. Of note, CD16 surface expression levels, pro-apoptotic and activation markers, and viability of patient and healthy donor NK cell subpopulations were not affected by this treatment. Accordingly, cetuximab restored killing activity of sMICA-inhibited patient NK cells against cetuximab-coated primary HNSCC cells via ADCC in a dose dependent manner. Rapid reconstitution of anti-tumor recognition and enhanced tumor infiltration of treated NK cells was monitored by 24 h co-incubation of HNSCC tumor spheroids with cetuximab (1 mu g/ml) and was characterized by increased IFN-gamma and INF alpha. secretion. This data show that the impaired NK cell-dependent tumor surveillance in relapsed HNSCC patients could be reversed by the re-establishment of ADCC-mediated effector cell activity, thus supporting NK cell-based immunotherapy in combination with antineoplastic monoclonal mAbs.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Kloess, Stephan UNSPECIFIED UNSPECIFIED UNSPECIFIED Chambron, Nicole UNSPECIFIED UNSPECIFIED UNSPECIFIED Gardlowski, Tanja UNSPECIFIED UNSPECIFIED UNSPECIFIED Weil, Sandra UNSPECIFIED UNSPECIFIED UNSPECIFIED Koch, Joachim UNSPECIFIED UNSPECIFIED UNSPECIFIED Esser, Ruth UNSPECIFIED UNSPECIFIED UNSPECIFIED von Strandmann, Elke Pogge UNSPECIFIED UNSPECIFIED UNSPECIFIED Morgan, Michael A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Arseniev, Lubomir UNSPECIFIED UNSPECIFIED UNSPECIFIED Seitz, Oliver UNSPECIFIED UNSPECIFIED UNSPECIFIED Koehl, Ulrike UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-387283
DOI:	10.3389/fimmu.2015.00543
Journal or Publication Title:	Front. Immunol.
Volume:	6
Date:	2015
Publisher:	FRONTIERS MEDIA SA
Place of Publication:	LAUSANNE
ISSN:	1664-3224
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language NATURAL-KILLER-CELL; ACTIVATING RECEPTOR NKG2D; PROTEIN-TYROSINE KINASES; LUNG-CANCER; NECK-CANCER; PHASE-II; EFFECTOR FUNCTIONS; REFERENCE VALUES; DENDRITIC CELLS; DOWN-REGULATION Multiple languages Immunology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/38728